Polysaccharide Extraction from Lemna Microcrop
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Solution Overview
Problem
There is a need for improved methods and systems to produce carbohydrate-rich products and polysaccharide products, such as apiogalacturonan, oligogalacturonide, and polygalacturonide, that require reduced water and energy expenditures, particularly in regions with water conservation concerns.
Innovation Solution
A method involving the treatment of microcrop biomass, including lysing, separating, and extracting using acid or oxalic acid solutions, followed by concentration processes like evaporation or ultrafiltration to produce polysaccharide products with high purity and yield, specifically utilizing Lemna species for apiogalacturonan, oligogalacturonide, and polygalacturonide extraction.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If conventional polysaccharide extraction methods are used, then polysaccharide products can be obtained, but water and energy consumption is high
Solution Approach 1:
The patent employs parameter changes by adjusting pH levels during extraction, using acid solutions (oxalic acid, hydrochloric acid) to optimize polysaccharide solubility and recovery. Temperature parameters are also controlled during extraction and concentration processes to enhance yield while managing energy consumption. The extraction solution composition and processing conditions are systematically optimized to achieve high polysaccharide recovery with reduced resource input.
Solution Approach 2:
The core of this invention is the extraction process itself - selectively extracting polysaccharides from microcrop biomass using acid solutions. The method takes out the desired polysaccharide components (apiogalacturonan, oligogalacturonide, polygalacturonide) from the complex biomass matrix through controlled chemical extraction, followed by separation and concentration to obtain purified products with high yield and purity.
2Productivity
If extraction efficiency is increased to produce more polysaccharide products, then carbohydrate sources become more available, but water and energy expenditures increase
Solution Approach 1:
The patent applies preliminary action through pre-treatment of the microcrop biomass before extraction. The biomass undergoes preparation steps (such as drying, grinding, or pre-extraction treatments) that optimize its structure for subsequent polysaccharide extraction. This preliminary processing enhances the efficiency of the main extraction step, allowing for higher productivity while potentially reducing the total energy and water required for the complete process.
Solution Approach 2:
The extraction process is designed to maintain continuous useful action through optimized process flow. The use of acid solutions allows for efficient solubilization of polysaccharides, followed by continuous separation and concentration steps. The method ensures that each stage of processing builds on the previous stage without significant losses, maintaining high productivity while minimizing redundant energy and water consumption throughout the process.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method efficiently produces polysaccharide products with high purity and yield, reducing water and energy consumption, and is suitable for regions with water conservation challenges, providing a sustainable source of carbohydrate-rich materials.
Implementation Method 1
extracting the first solid with an extraction solution to form a polysaccharide extract
Implementation Method 2
concentrating a polysaccharide extract to form a polysaccharide product
Data Source
AI summary
The present disclosure relates, according to some embodiments, to methods and systems for purifying proteins, carbohydrate rich products, and polysaccharide products from a microcrop (e.g., photosynthetic aquatic species) and compositions thereof. For example, the present disclosure relates, in some embodiments to methods and systems for extracting proteins, dry biocrude, carbohydrate-rich meal, and polysaccharide products from Lemna.