Lettuce E01G.11092 Breeding via Molecular Markers

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Solution Overview

Problem

There is a need for new lettuce varieties that are stable, high-yielding, and agronomically sound to meet increasing global demand, with a requirement for improved traits such as disease resistance, pest resistance, and adaptability to various environmental conditions.

Innovation Solution

The development of the Lactuca sativa variety 'E01G.11092' with specific traits like immunity to certain diseases and pests, combined with methods for introducing desirable genes for herbicide resistance, pest resistance, and disease resistance through breeding techniques such as backcrossing and genetic marker-enhanced selection, along with tissue culture for regeneration.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional breeding methods are used to develop new lettuce varieties, then genetic diversity and adaptability are improved, but breeding time and complexity increase

Engineering Contradiction:
Improveadaptability to environmental conditionsVSAvoidbreeding time
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by using molecular markers to pre-identify plants carrying desired resistance genes before actual breeding crosses are made. This allows breeders to select parent plants with specific traits in advance, significantly reducing the time required for traditional phenotypic screening and accelerating the breeding process while maintaining genetic diversity and adaptability.

Inventive Principle:
Principle #10Preliminary action

2Manufacturing precision

If molecular marker-enhanced selection is applied, then breeding precision and trait introduction efficiency are improved, but technical complexity and cost increase

Engineering Contradiction:
Improvebreeding precisionVSAvoidtechnical complexity
Core Design Contradiction:
Manufacturing precisionVSDevice complexity

Solution Approach 1:

The patent uses molecular markers as intermediaries to bridge the gap between desired traits and selectable phenotypes. These markers serve as reliable indicators that allow breeders to indirectly select for complex traits like disease resistance without directly observing them, thereby achieving high breeding precision while managing technical complexity through established molecular biology techniques.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Stability of the object's composition

If backcrossing methods are used to introduce specific traits, then trait purity and stability are improved, but number of generations and time required increase

Engineering Contradiction:
Improvetrait stabilityVSAvoidnumber of generations
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

The patent implements feedback by using molecular markers to continuously monitor and track the presence of desired traits and the elimination of unwanted genetic material throughout the backcrossing process. This real-time genetic monitoring allows breeders to make informed decisions at each generation, accelerating the recovery of the recurrent parent genome while ensuring trait stability, thereby reducing the number of generations needed compared to traditional methods.

Inventive Principle:
Principle #23Feedback

4Reliability

If new lettuce varieties with disease resistance are developed, then crop reliability and yield stability are improved, but breeding program complexity and resource requirements increase

Engineering Contradiction:
Improvedisease resistanceVSAvoidbreeding program complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent applies segmentation by breaking down the complex task of developing disease-resistant lettuce varieties into manageable components: (1) identifying molecular markers associated with resistance, (2) selecting parent plants with desired markers, (3) performing targeted crosses, and (4) tracking marker inheritance. This segmented approach allows breeding programs to systematically address disease resistance while reducing overall program complexity and resource requirements.

Inventive Principle:
Principle #1Segmentation

Data Source

PatentUS12089549B2Lettuce variety ‘E01G.11092’
Publication Date: 2024.09.17 ENZA ZADEN BEHEER BV
  • US12089549B2 patent drawing
  • US12089549B2 patent drawing
  • US12089549B2 patent drawing

AI summary

New lettuce variety designated ‘E01G.11092’ is described. ‘E01G.11092’ is a lettuce variety exhibiting stability and uniformity.