Iceberg Lettuce Line Breeding for Uniform Mildew-Resistant Progeny

Resolve Bottlenecks,
Find Innovative Solutions
Generate Solutions

Solution Overview

Problem

Existing lettuce breeding methods struggle to produce uniform varieties with desirable traits such as resistance to diseases and pests, as well as improved agronomic qualities, due to genetic non-uniformity in hybrid plants.

Innovation Solution

Development of the iceberg lettuce cultivar NNS-2117, which is homozygous and uniform, achieved through selective breeding and potentially enhanced with traits like herbicide tolerance, insect resistance, and disease resistance via backcrossing and genetic transformation, ensuring genetic stability and uniformity.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If cross-pollination is used to create hybrid plants, then genetic diversity and trait combination are improved, but genetic uniformity and predictability deteriorate

Engineering Contradiction:
Improvegenetic diversityVSAvoidgenetic uniformity
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The breeding program segments the hybridization process into distinct generations: F1 hybrids provide genetic diversity, while subsequent self-pollination segments the population into homozygous lines that can be selected for uniformity. This allows sequential achievement of both diversity and uniformity at different stages.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent applies preliminary action by creating and evaluating multiple F1 hybrid combinations before selecting the best performing cross. This preliminary hybridization step allows identification of parent combinations that will produce uniform, high-performing progeny, resolving the contradiction between diversity exploration and uniformity selection.

Inventive Principle:
Principle #10Preliminary action

2Stability of the object's composition

If self-pollination is used to create homozygous lines, then genetic uniformity is improved, but genetic diversity and trait combination worsen

Engineering Contradiction:
Improvegenetic uniformityVSAvoidgenetic diversity
Core Design Contradiction:
Stability of the object's compositionVSAdaptability or versatility

Solution Approach 1:

The patent merges multiple homozygous lines through controlled cross-pollination to create F1 hybrids that combine the genetic uniformity of inbred parents with the genetic diversity of hybrid progeny. This merging resolves the contradiction by achieving both uniformity (in the parental lines) and diversity (in the hybrid combination).

Inventive Principle:
Principle #5Merging (Combining)

3Manufacturing precision

If extensive breeding evaluation is conducted to select uniform varieties, then variety quality is improved, but breeding time and complexity worsen

Engineering Contradiction:
Improvevariety uniformityVSAvoidbreeding duration
Core Design Contradiction:
Manufacturing precisionVSLoss of time

Solution Approach 1:

The patent evaluates a limited number of selected F1 hybrid combinations rather than all possible crosses, applying partial action to focus resources on the most promising parent pairs. This reduces breeding time while still achieving uniform variety selection by concentrating evaluation efforts where they are most likely to succeed.

Inventive Principle:
Principle #16Partial or excessive action

Solution Approach 2:

The breeding program extracts and eliminates non-performing hybrid combinations early in the evaluation process, removing unpromising lines from further consideration. This extraction of inferior options reduces the overall breeding timeline while maintaining the selection of uniform, high-quality varieties.

Inventive Principle:
Principle #2Taking out (Extraction)

Data Source

PatentUS20250366425A1Iceberg Lettuce Cultivar NNS-2117
Publication Date: 2025.12.04 NIPOMO NATIVE SEEDS LLC
  • US20250366425A1 patent drawing
  • US20250366425A1 patent drawing
  • US20250366425A1 patent drawing

AI summary

The disclosure provides seed and plants of the iceberg lettuce cultivar designated cv. NNS-2117. The disclosure thus relates to the plants, seeds and tissue cultures of iceberg lettuce cv. NNS-2117, and to methods for producing a lettuce plant produced by crossing a plant of iceberg lettuce cv. NNS-2117 with itself or with another lettuce plant, such as a plant of another line. The disclosure further relates to seeds and plants produced by such crossing. The disclosure further relates to parts of a plant of iceberg lettuce line cv. NNS-2117, including the gametes of such plants.