Lettuce Variety MULTIGREEN 57 Genetic Transformation

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Solution Overview

Problem

The demand for new lettuce varieties with specific traits such as colorful and interesting shapes, sizes, textures, and high yield has increased, particularly for use as garnishes and in processed salads, which existing breeding methods struggle to meet effectively.

Innovation Solution

Development of a new lettuce variety, MULTIGREEN 57, characterized by distinct morphological and physiological features like dark green color, pinnately lobed shape, and enhanced resistance to pests and diseases, achieved through breeding and genetic transformation methods, including crossing, selfing, and introducing single locus conversions or transgenic traits.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional breeding methods are used to develop lettuce varieties, then basic yield and adaptability are maintained, but the ability to meet specific market demands for colorful shapes, sizes, and textures is insufficient

Engineering Contradiction:
Improveadaptability to specific market demandsVSAvoidyield
Core Design Contradiction:
Adaptability or versatilityVSProductivity

Solution Approach 1:

The patent employs genetic transformation to introduce specific transgenic traits that modify plant parameters such as leaf color, shape, size, and texture. This allows precise adjustment of phenotypic parameters to meet diverse market requirements while maintaining high yield potential through controlled genetic modification rather than traditional slow breeding methods.

Inventive Principle:
Principle #35Parameter changes

2Adaptability or versatility

If new traits are introduced through genetic transformation, then market demand requirements are met, but the complexity of the breeding process increases

Engineering Contradiction:
Improvespecific traitsVSAvoidbreeding process complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent utilizes preliminary action by first developing and characterizing specific transgenic traits in controlled conditions, then systematically introducing these pre-tested traits into breeding programs. This preliminary preparation of genetic material and trait validation reduces the overall complexity of the breeding process by avoiding trial-and-error approaches.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent employs molecular markers as intermediaries to track and select for desired transgenic traits during breeding. These markers serve as reliable indicators that simplify the selection process, allowing breeders to efficiently identify plants with target traits without complex phenotypic analysis, thereby reducing breeding program complexity.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Reliability

If extensive breeding and evaluation are conducted to develop new cultivars, then desirable traits are combined, but the time required for cultivar development increases

Engineering Contradiction:
Improvedesirable traits combinationVSAvoidcultivar development time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent replaces traditional mechanical breeding methods (manual crossing, phenotypic selection) with molecular biology techniques including DNA-based marker-assisted selection and transgenic trait introduction. This substitution accelerates the breeding process by enabling direct genetic manipulation and precise trait selection, reducing development time from years to months while maintaining reliable combination of desirable traits.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Data Source

PatentUS9198395B2Lettuce variety multigreen 57 LTL
Publication Date: 2015.12.01 NUNHEMS BV
  • US9198395B2 patent drawing
  • US9198395B2 patent drawing

AI summary

The present invention relates to plants of a lettuce variety MULTIGREEN 57 (also referred to as “NUN 09057” or “09057” or NUN 09057 LTL) and, seeds and progeny thereof. The invention further relates to methods for producing a lettuce plant by traditional breeding methods. Moreover, the invention relates to a method for producing a lettuce plant containing in its genetic material one or more transgenes.