Lettuce Line P2231-3013596 Self-Pollination Breeding
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Solution Overview
Problem
Current lettuce breeding methods face challenges in developing uniform and stable varieties due to self-pollination, low seed production, and the tedious nature of manual crossing, which limits the introduction of desirable traits such as herbicide tolerance, insect resistance, and improved nutritional value.
Innovation Solution
The development of the lettuce line P2231-3013596, which is homozygous and self-pollinating, allowing for the introduction of desired traits through genetic transformation or backcrossing, and the production of hybrid seeds with specific characteristics like resistance to corky root rot and tall, wide heads, using techniques like pedigree selection and tissue culture for regenerable cells.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If manual crossing is used for hybrid development, then genetic diversity can be introduced, but the process becomes tedious and seed production per cross is low
Solution Approach 1:
The patent utilizes lettuce's natural self-pollination capability to produce uniform inbred lines without requiring manual emasculation or crossing. The plant serves itself by transferring its own pollen, eliminating the tedious manual operations while maintaining genetic control through selective breeding of self-pollinating lines.
Solution Approach 2:
The patent performs preliminary selection of desirable traits within inbred lines before hybridization. By pre-selecting parent lines with specific desired characteristics through self-pollination and selection over generations, the actual hybrid crossing becomes more efficient and targeted, improving seed production relevance.
2Stability of the object's composition
If self-pollination is utilized for breeding, then uniform and vigorous inbred lines are produced, but the introduction of new traits becomes limited
Solution Approach 1:
The patent segments the breeding process into distinct phases: first developing uniform inbred lines through self-pollination, then introducing specific traits through targeted backcrossing or genetic transformation. This segmentation allows maintaining genetic uniformity in the base line while separately introducing desired traits without disrupting the overall uniformity.
Solution Approach 2:
The patent uses backcrossing as an intermediary method to introduce new traits into established inbred lines. By crossing the inbred line with a donor line carrying desired traits and then backcrossing to the original inbred line, new traits are introduced while maintaining the genetic uniformity and background of the original line.
3Reliability
If pedigree breeding and backcrossing are used, then desirable traits can be improved, but the process requires waiting for flower opening and emasculation which is tedious and difficult
Solution Approach 1:
The patent exploits lettuce's natural self-pollination mechanism, allowing the plant to perform the pollination function itself without human intervention for emasculation or pollen transfer. This eliminates the tedious and difficult manual operations while maintaining reliable trait inheritance through controlled selection of self-pollinating lines.
4Adaptability or versatility
If hybrid development is pursued, then genetic diversity and trait variation are increased, but the process is tedious with low seed production
Solution Approach 1:
The patent leverages lettuce's natural self-pollination to produce seeds efficiently without requiring manual hybridization operations. By developing uniform inbred lines that self-pollinate reliably, the system achieves high seed production efficiency while maintaining genetic diversity through the selection and combination of different inbred lines with desirable traits.
Data Source
AI summary
The invention provides seed and plants of the lettuce line designated P2231-3013596. The invention thus relates to the plants, seeds and tissue cultures of lettuce line P2231-3013596, and to methods for producing a lettuce plant produced by crossing a plant of lettuce line P2231-3013596 with itself or with another lettuce plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of lettuce line P2231-3013596, including the gametes of such plants.