Lettuce Line PS06519291 Self-Pollination Breeding
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Solution Overview
Problem
Current lettuce breeding methods face challenges in developing uniform and stable varieties due to self-pollination, low seed production, and the tedious nature of manual crossing, which limits the introduction of desirable traits such as herbicide tolerance, insect resistance, and improved carbohydrate metabolism.
Innovation Solution
The development of the lettuce line PS06519291, which is homozygous and self-pollinated, combined with genetic transformation techniques to introduce desired traits like herbicide tolerance and resistance, and a process for producing hybrid seeds through controlled pollination methods, ensuring uniformity and stability in seed yield.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If manual crossing is used for hybrid development, then genetic diversity can be introduced, but the process becomes tedious and seed production per cross is low
Solution Approach 1:
The patent utilizes lettuce's natural self-pollination capability to propagate homozygous inbred lines without requiring manual intervention for pollination. This self-service mechanism maintains genetic uniformity while eliminating the need for tedious manual crossing, thereby resolving the contradiction between introducing genetic diversity and maintaining productivity.
2Stability of the object's composition
If self-pollination is utilized for breeding, then uniform and vigorous inbred lines are produced, but the ability to introduce new traits through cross-pollination is limited
Solution Approach 1:
The patent employs preliminary action by first developing stable homozygous inbred lines through self-pollination, then using these lines as parents for controlled cross-pollination. This preliminary establishment of uniform genetic backgrounds enables subsequent introduction of desirable traits through crossing while maintaining the benefits of genetic uniformity in the base population.
3Reliability
If pedigree breeding and backcrossing are used, then desirable traits can be improved, but the process is time-consuming and reduces productivity
Solution Approach 1:
The patent applies partial action by selecting specific desirable traits for improvement through backcrossing rather than attempting to improve all traits simultaneously. This focused approach on key traits reduces the number of breeding cycles required, thereby improving breeding efficiency and productivity while still achieving reliable trait improvement.
4Ease of operation
If emasculation is performed before crossing, then cross-pollination can be controlled, but the process becomes more difficult and time-consuming
Solution Approach 1:
The patent extracts the self-pollen from flowers by washing it away, eliminating the need for complex emasculation procedures. This simplified approach maintains control over cross-pollination while reducing operational difficulty and time requirements, thereby resolving the contradiction between crossing control and ease of operation.
Data Source
AI summary
The invention provides seed and plants of the lettuce line designated PS06519291. The invention thus relates to the plants, seeds and tissue cultures of lettuce line PS06519291, and to methods for producing a lettuce plant produced by crossing a plant of lettuce line PS06519291 with itself or with another lettuce plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of lettuce line PS06519291, including the gametes of such plants.