Lettuce Cultivar SPRITZER Breeding via Molecular Markers

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Solution Overview

Problem

The development of new lettuce cultivars is a time-consuming process that requires precise planning and significant resources, with challenges in identifying genetically superior individuals due to confounding traits and environmental factors, leading to unpredictability and high research costs.

Innovation Solution

The introduction of novel lettuce cultivars SCARAMANGA, GIMLET, SPRITZER, and MOJITO, which are developed through advanced breeding techniques including pedigree selection, recurrent selection, and molecular marker-assisted selection, incorporating traits such as disease resistance, drought tolerance, and improved shelf-life, and can be used to produce transgenic plants with specific traits like male sterility and herbicide resistance.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional breeding methods are used to develop new lettuce cultivars, then genetic diversity and trait improvement can be achieved, but the process becomes time-consuming and unpredictable due to confounding traits and environmental factors

Engineering Contradiction:
Improvepredictability of breeding outcomesVSAvoidbreeding development time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent replaces traditional mechanical/phenotypic selection methods with molecular marker-assisted selection. Instead of visually evaluating plant traits in the field (mechanical observation), the invention uses DNA-based molecular markers to identify and select plants with desired genetic traits, thereby reducing the time and increasing the reliability of breeding outcomes.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent introduces molecular markers as an intermediary between the breeder and the plant's genetic traits. These markers serve as proxies or indicators that allow breeders to indirectly detect and select for desirable traits without having to wait for phenotypic expression or deal with environmental confounding factors.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Productivity

If traditional breeding methods are used to develop new lettuce cultivars, then new varieties can be produced, but research costs increase due to unpredictability and resource requirements

Engineering Contradiction:
Improvecultivar development efficiencyVSAvoidresearch costs
Core Design Contradiction:
ProductivityVSLoss of energy

Solution Approach 1:

The patent performs preliminary genetic analysis using molecular markers before conducting full-scale breeding trials. By identifying plants with desired genetic markers early in the breeding process, researchers can prioritize which plants to advance to the next generation, thereby reducing the overall number of plants and resources that need to be managed throughout the breeding program.

Inventive Principle:
Principle #10Preliminary action

3Measurement precision

If molecular marker-assisted selection is implemented, then breeding precision and reliability improve, but device and method complexity increases

Engineering Contradiction:
Improvegenetic trait detection accuracyVSAvoidbreeding methodology complexity
Core Design Contradiction:
Measurement precisionVSDevice complexity

Solution Approach 1:

The patent segments the complex breeding process into distinct phases: molecular marker analysis phase, selection phase, and traditional breeding phase. By separating the genetic analysis component from the physical breeding operations, the system achieves high measurement precision for genetic traits while keeping the overall breeding program manageable through modular, staged implementation.

Inventive Principle:
Principle #1Segmentation

Data Source

PatentUS9609843B2Lettuce named spritzer
Publication Date: 2017.04.04 VILMORIN MIKADO USA INC

AI summary

Novel lettuce cultivar, designated SPRITZER is disclosed. The invention relates to the seeds of lettuce cultivar SPRITZER, to the plants of lettuce line SPRITZER and to methods for producing a lettuce plant by crossing the cultivar SPRITZER with itself or another lettuce line, respectively. The invention further relates to methods for producing a lettuce plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other lettuce lines derived from the cultivar SPRITZER.