Leuconostoc HX-236 Fermentation for Cordyceps Militaris Active Release

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Solution Overview

Problem

Current anti-aging cosmetics lack efficacy in improving cellular hypoxia and addressing skin aging issues such as decreased cell vitality, collagen loss, pigmentation, and reduced skin elasticity, while existing Cordyceps militaris products do not fully release their active ingredients.

Innovation Solution

The use of Leuconostoc sp. HX-236 for fermenting Cordyceps militaris to enhance the release of active ingredients like cordycepin and cordycepic acid, resulting in a fermentation product that regulates skin inflammation, promotes collagen endogeny, and improves skin autophagy, thereby addressing skin aging.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Quantity of substance

If conventional anti-aging cosmetics are used to provide skin hydration, then skin hydration is improved, but efficacy in improving cellular hypoxia and anti-aging is insufficient

Engineering Contradiction:
Improveskin hydrationVSAvoidanti-aging efficacy
Core Design Contradiction:
Quantity of substanceVSReliability

Solution Approach 1:

The patent changes the chemical composition parameters of the fermentation medium, using Cordyceps militaris as the sole nitrogen source and optimizing carbon source composition (glucose, fructose, sucrose in specific ratios) to enhance the production of bioactive compounds with anti-aging properties while maintaining hydration benefits

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent creates a composite fermentation product containing multiple active ingredients (cordycepin, cordycepic acid, peptides, extracellular polysaccharides) that work synergistically to provide both hydration and anti-aging effects, resolving the contradiction between hydration and anti-aging efficacy

Inventive Principle:
Principle #40Composite materials

2Reliability

If Cordyceps militaris is used as a raw material for skin care, then antioxidation and antibacterial effects are improved, but active ingredients are not fully released

Engineering Contradiction:
Improveantioxidation effectVSAvoidactive ingredient release
Core Design Contradiction:
ReliabilityVSQuantity of substance

Solution Approach 1:

The patent introduces lactic acid bacteria as an intermediary organism that ferments Cordyceps militaris, breaking down cell walls and releasing bound active ingredients through enzymatic action, thereby significantly increasing the availability of cordycepin, cordycepic acid, and other bioactive compounds

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent replaces mechanical extraction methods with biological fermentation, using lactic acid bacteria enzymes to naturally break down and release active ingredients from Cordyceps militaris, achieving more complete extraction without mechanical disruption

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

3Quantity of substance

If lactic acid bacteria fermentation is applied to Cordyceps militaris, then content of active ingredients is increased, but manufacturing complexity increases

Engineering Contradiction:
Improveactive ingredient contentVSAvoidfermentation process complexity
Core Design Contradiction:
Quantity of substanceVSDevice complexity

Solution Approach 1:

The patent employs lactic acid bacteria that naturally occur in the environment to perform self-fermentation of Cordyceps militaris, utilizing the bacteria's inherent enzymatic systems to break down and release active ingredients without requiring complex external intervention or sophisticated fermentation equipment

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The patent optimizes fermentation parameters (temperature, pH, incubation time, bacterial inoculum size) to achieve maximum active ingredient release under simple, controllable conditions, reducing manufacturing complexity while maintaining high efficacy

Inventive Principle:
Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The Cordyceps militaris fermentation product with Leuconostoc sp. HX-236 increases skin collagen I expression by 2-8%, enhances cell vitality, and reduces inflammation, effectively improving skin elasticity and reducing aging signs.

Implementation Method 1

after fermentation with lactic acid bacteria, the content of active ingredients in Cordyceps militaris can be further increased

Methodology Applied
Scientific EffectFermentation: Fermentation

Data Source

PatentEP4644530A1Leuconostoc and use thereof
Publication Date: 2025.11.05 BLOOMAGE BIOTECHNOLOGY CORP LTD
  • EP4644530A1 patent drawingFigure 1A~3C
  • EP4644530A1 patent drawingFigure 4
  • EP4644530A1 patent drawing

AI summary

The present application discloses leuconostoc HX-236 and a use thereof. HX-236 takes Cordyceps militaris powder as a unique nitrogen source, and a fermentation filtrate can be obtained by means of fermentation culture. The fermentation filtrate comprises high contents of cordycepin and cordycepic acid, and can improve the expression of hypoxia-inducible factors (HIFs) 1α in cells, so that downstream signaling pathways for regulation of inflammatory response, cell autophagy, and tissue regeneration are activated, thereby alleviating the problems of skin aging such as cell viability decline, collagen loss, pigmentation, and skin elasticity reduction caused by hypoxia of the skin.