Conjugate Composition for LH Peak Detection
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Solution Overview
Problem
Current ovulation detection methods for mammals are not 100% reliable, often resulting in false positives and are costly, difficult to apply, and not directly applicable to various mammalian species due to specific antibody requirements, making it challenging to accurately detect the preovulatory LH peak.
Innovation Solution
A conjugate composition comprising an antibody coupled to an enzyme, fetal calf serum, and glycerol, with varying concentrations of normal rabbit serum and glycerol, which can be stored at refrigerated temperatures without denaturation, enhancing sensitivity and stability for detecting LH peaks in biological samples across different mammalian species.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional ovulation detection methods are used, then detection can be performed, but false positives occur and reliability is reduced
Solution Approach 1:
The patent optimizes the concentration parameters of fetal calf serum (5-50%) and glycerol (1-30%) in the conjugate composition to achieve optimal detection performance. By adjusting these compositional parameters, the test achieves high reliability in detecting the preovulatory LH peak while minimizing false positives, resolving the contradiction between reliability and measurement precision.
Solution Approach 2:
The patent creates a composite conjugate composition combining antibody-enzyme conjugates with fetal calf serum and glycerol. This composite formulation enhances the stability and sensitivity of the detection system, improving both reliability and precision simultaneously by synergistically combining multiple components in optimized proportions.
2Reliability
If species-specific antibodies are used for different mammalian species, then detection accuracy is maintained, but cost and complexity increase
Solution Approach 1:
The patent develops a universal conjugate composition based on fetal calf serum and glycerol that can be used across multiple mammalian species (humans, cows, pigs, sheep, goats, cats, dogs). This single formulation serves multiple species simultaneously, eliminating the need for species-specific antibody formulations and reducing both cost and complexity while maintaining detection accuracy.
3Stability of the object's composition
If conventional reagent storage conditions are used, then storage is simple, but biological activity is lost due to denaturation
Solution Approach 1:
The patent introduces fetal calf serum and glycerol as protective intermediary substances in the conjugate composition. These components act as stabilizers that prevent denaturation of the antibody-enzyme conjugates during storage. The glycerol specifically provides cryoprotection and maintains molecular integrity, extending shelf life while preserving biological activity under refrigerated storage conditions.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The composition increases the sensitivity and reliability of LH peak detection, reduces background noise, and allows for simple interpretation, making it suitable for various mammals while maintaining biological activity and cost-effectiveness.
Implementation Method 1
an antibody coupled to an enzyme, fetal calf serum, 5 to 50% by volume of serum normal rabbit relative to the total volume of the composition
Implementation Method 2
from 1 to 30% by volume relative to the total volume of the glycerol composition... can be stored at refrigerated temperatures without denaturation
Implementation Method 3
increases the sensitivity and reliability of LH peak detection, reduces background noise
Data Source
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AI summary
The present invention relates to a composition of conjugates and its use in a method of detecting compounds, for example biological molecules, for example peptides, proteins, hormones, nucleic acids, antibodies in a sample, for example a biological sample. The present invention relates in particular to a composition of conjugates and its use in a method of detecting the pre-ovulatory spike in LH in a biological sample originating from mammals and a kit for implementing the method of detection. The present invention finds an application in particular in the veterinary and medical fields.