Lichenysin Lipopeptide Volatility Control in Chinese Spirits
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Solution Overview
Problem
Current research on alcoholic beverages focuses primarily on volatile aroma compounds, neglecting the impact of nonvolatile matrix compounds, particularly in Chinese liquors, which significantly influence the aroma quality and consumer preferences.
Innovation Solution
The method involves adding lichenysin to Chinese liquors at specific concentrations, followed by purification and quantification using SPE C18 cartridges and UPLC-MS, enhancing the aroma by affecting the volatility of aroma and off-flavor compounds through HS-SPME-GC-MS analysis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If research focuses on volatile aroma compounds, then aroma identification is improved, but understanding of matrix compound effects is insufficient
Solution Approach 1:
The patent segments the aroma research into two distinct parts: volatile aroma compounds and nonvolatile matrix compounds. By separately analyzing each component using appropriate methods (GC-MS for volatile compounds and UPLC-MS for matrix compounds), the study comprehensively identifies both aroma substances and their interacting matrix compounds, preventing loss of information about compound interactions.
Solution Approach 2:
The patent introduces UPLC-MS as an intermediary tool to detect and analyze nonvolatile matrix compounds that interact with aroma compounds. This intermediary method bridges the gap between studying volatile aroma compounds and understanding their interactions with matrix compounds, enabling comprehensive aroma quality assessment.
2Quantity of substance
If conventional methods are used to study aroma compounds, then volatile compounds are identified, but nonvolatile macrocompounds remain poorly understood
Solution Approach 1:
The patent replaces conventional GC-MS methodology (designed for volatile compounds) with UPLC-MS technology specifically suited for nonvolatile macrocompounds. This substitution enables detection and quantification of lipopeptides and other matrix compounds that were previously difficult or impossible to measure, overcoming the limitations of conventional methods.
3Manufacturing precision
If lipopeptides are added to improve aroma, then aroma quality is enhanced, but precise quantification and control are challenging
Solution Approach 1:
The patent establishes a feedback loop where UPLC-MS quantifies lipopeptide content in the liquor, and this quantitative data feeds back to adjust production parameters and addition amounts. This closed-loop system enables precise control of lipopeptide levels to optimize aroma quality while maintaining consistent product standards.
Solution Approach 2:
The patent utilizes UPLC-MS to detect specific parameter changes in lipopeptide concentration, molecular weight distribution, and composition ratios. By monitoring these parameter changes, the production process can precisely control lipopeptide addition to achieve desired aroma effects while maintaining quality consistency.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Significantly improves the aroma quality and reduces the volatility of phenolic off-flavors while enhancing the volatility of esters, alcohols, and acids, as demonstrated by sensory evaluation and chromatographic analysis.
Implementation Method 1
affecting the volatility of aroma and off-flavor compounds through HS-SPME-GC-MS analysis
Implementation Method 2
purification and quantification using SPE C18 cartridges
Implementation Method 3
UPLC-MS
Implementation Method 4
headspace solid phase micro extraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS)
Data Source
Figure 1~2
Figure 3
Figure 4(A)
AI summary
Disclosed is the use of a lipopeptide compound for improving or increasing the aroma of beverages, wherein the beverages are alcoholic, such as Chinese distillate spirits. Also disclosed is a method for preparing a lipopeptide compound, characterized in that separation and purification are performed using distillate spirits. Also disclosed is a method for determining the content of a lipopeptide compound in distillate spirits, wherein the method combines ultra-high performance liquid chromatography with mass spectrometry. The lipopeptide compound is selected from surfactin, lichenysin, iturin and fengycin.