Light-Blocking Vessel Insert for Precise Fluorescence Observation
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Solution Overview
Problem
Existing observation methods using opaque vessels for fluorescence or luminescent measurements face challenges in achieving accurate measurements with low light intensity and limited biological samples, while transparent vessels hinder precise fluorescence/luminescence observations.
Innovation Solution
An auxiliary member with a light-blocking portion that adheres to the bottom surface of a vessel compartment, allowing for both transmitted light cell observation and precise fluorescence/luminescence observation by fitting to a vessel with transparent bottom and opaque walls.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If an opaque vessel is used for fluorescence or luminescent measurement, then light diffusion is reduced and measurement precision is improved, but transmitted light observation cannot be performed
Solution Approach 1:
The vessel is divided into two distinct parts: the wall surface made of light-blocking material (black) and the bottom surface made of light-transmitting material (transparent). This segmentation allows each part to fulfill its specific function - the walls block light to prevent diffusion during fluorescence/luminescence measurement, while the bottom transmits light for bright-field observation, thus resolving the contradiction between measurement precision and observation versatility
Solution Approach 2:
Different parts of the vessel are given different optical properties: the wall surfaces have light-blocking properties to suppress intrinsic fluorescence and prevent light diffusion, while the bottom surface has light-transmitting properties to enable transmitted light observation. This local differentiation of material properties allows the vessel to support both fluorescence/luminescence measurement and bright-field observation without compromise
2Adaptability or versatility
If a transparent vessel is used for transmitted light observation, then observation versatility is improved, but fluorescence/luminescence measurement precision deteriorates due to light diffusion
Solution Approach 1:
The vessel is divided into two distinct parts: the wall surface made of light-blocking material (black) and the bottom surface made of light-transmitting material (transparent). This segmentation allows each part to fulfill its specific function - the walls block light to prevent diffusion during fluorescence/luminescence measurement, while the bottom transmits light for bright-field observation, thus resolving the contradiction between measurement precision and observation versatility
Solution Approach 2:
Different parts of the vessel are given different optical properties: the wall surfaces have light-blocking properties to suppress intrinsic fluorescence and prevent light diffusion, while the bottom surface has light-transmitting properties to enable transmitted light observation. This local differentiation of material properties allows the vessel to support both fluorescence/luminescence measurement and bright-field observation without compromise
3Measurement precision
If an opaque vessel with opaque bottom is used for fluorescence measurement, then measurement precision is improved, but transmitted light observation is completely blocked
Solution Approach 1:
The vessel is divided into two distinct parts: the wall surface made of light-blocking material (black) and the bottom surface made of light-transmitting material (transparent). This segmentation allows each part to fulfill its specific function - the walls block light to prevent diffusion during fluorescence/luminescence measurement, while the bottom transmits light for bright-field observation, thus resolving the contradiction between measurement precision and observation versatility
Solution Approach 2:
Different parts of the vessel are given different optical properties: the wall surfaces have light-blocking properties to suppress intrinsic fluorescence and prevent light diffusion, while the bottom surface has light-transmitting properties to enable transmitted light observation. This local differentiation of material properties allows the vessel to support both fluorescence/luminescence measurement and bright-field observation without compromise
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables stable and precise fluorescence/luminescence observation by stabilizing the vessel orientation and reducing light diffusion, enhancing measurement accuracy with low light intensity samples.
Implementation Method 1
a light-blocking portion (11) that adheres to the bottom surface when the auxiliary member (1) is fitted to the vessel (9)
Data Source
AI summary
An auxiliary member that is attachable to and detachable from a vessel including a compartment whose bottom surface has a light transmission effect and whose wall surface has a light-blocking effect is used to perform fluorescence imaging or luminescent measurement on biological samples contained in the compartment. The auxiliary member includes a light-blocking portion that adheres to the bottom surface when the auxiliary member is fitted to the vessel. Accordingly, cell observation using transmitted light can be performed when the auxiliary member is not fitted to the vessel, and precise fluorescence/luminescence observation can be performed when the auxiliary member is fitted to the vessel. That is, it is possible to achieve both cell observation using transmitted light and precise fluorescence/luminescence observation when fluorescence imaging or luminescent measurement is performed on biological samples such as cells.