Lignocellulose Pretreatment via Colloid Mill and Homogenization

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Solution Overview

Problem

Current methods for processing lignocellulose materials are inefficient due to high costs, environmental pollution, and the need for complex equipment and high-temperature conditions, which hinder the production of fermentable sugars for biofuels and biochemicals.

Innovation Solution

A method involving crushing lignocellulose into granules, mixing with water, grinding with a colloid mill, high-pressure homogenization, and enzymolysis using cellulase, β-glucosidase, and xylanase to break down cellulose into fermentable sugars, avoiding acid and base treatments and high temperatures.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Ease of manufacture

If acid or alkali treatment methods are used to process lignocellulose, then the crystalline cellulose structure can be loosened for saccharification, but environmental pollution and high processing costs occur

Engineering Contradiction:
Improvesaccharification capabilityVSAvoidenvironmental pollution
Core Design Contradiction:
Ease of manufactureVSObject-affected harmful factors

Solution Approach 1:

The patent replaces chemical treatment methods (acid/alkali) with a physical-mechanical system consisting of a colloid mill and homogenizer. The colloid mill mechanically crushes lignocellulose particles, and the homogenizer applies high shear force and cavitation to disrupt the crystalline structure, achieving saccharification capability without chemical reagents that cause pollution.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent converts the naturally recalcitrant crystalline structure of cellulose, which normally resists enzymatic hydrolysis, into a benefit by using mechanical energy to deliberately disrupt this structure. The high shear force and cavitation effects break down the crystalline regions, increasing surface area and accessibility for enzymes, thus turning a barrier into an advantage for saccharification.

Inventive Principle:
Principle #22Blessing in disguise (Convert harm into benefit)

2Productivity

If nano-scale crushing is used to improve hydrolysis efficiency, then cellulose structure is better loosened, but energy consumption increases and temperature rise causes long intercooling time

Engineering Contradiction:
Improvehydrolysis efficiencyVSAvoidenergy consumption
Core Design Contradiction:
ProductivityVSUse of energy by moving object

Solution Approach 1:

The patent applies partial crushing action through the colloid mill followed by homogenization, rather than attempting to achieve complete nano-scale reduction. This staged approach provides sufficient surface area increase for effective hydrolysis without the excessive energy consumption and temperature rise associated with prolonged nano-scale grinding, optimizing the balance between productivity and energy use.

Inventive Principle:
Principle #16Partial or excessive action

Solution Approach 2:

The patent introduces water as an intermediary medium in the colloid mill processing. The wet milling approach allows for more efficient heat dissipation compared to dry milling, reducing temperature rise and subsequent cooling time. Water also facilitates the mechanical disruption of cellulose structure while maintaining lower energy requirements.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Ease of manufacture

If steam explosion or wet oxidation methods are used to process lignocellulose, then the crystalline structure is disrupted, but special explosion equipment is required and safety requirements are high

Engineering Contradiction:
Improvestructure disruption capabilityVSAvoidequipment complexity
Core Design Contradiction:
Ease of manufactureVSDevice complexity

Solution Approach 1:

The patent replaces complex thermal-explosion equipment with simpler mechanical processing equipment (colloid mill and homogenizer). The colloid mill performs initial particle size reduction, and the homogenizer delivers the structure-disrupting action through high shear force and cavitation, achieving comparable or superior effectiveness to steam explosion without the need for pressure vessels, steam generation systems, or safety interlocks.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The homogenizer utilizes the kinetic energy of the liquid stream and the geometry of the valve to generate cavitation and shear forces automatically during normal operation. The system self-generates the necessary disruptive forces without requiring external heating, pressurization, or complex control systems, thereby simplifying equipment while maintaining structure disruption capability.

Inventive Principle:
Principle #25Self-service

4Productivity

If organic solvent extraction is used to remove lignin after hydrolysis, then lignin separation is achieved, but additional neutralization and filtering steps are required

Engineering Contradiction:
Improvelignin separation efficiencyVSAvoidprocess steps
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent replaces chemical extraction methods with mechanical separation approaches. The colloid mill and homogenizer mechanically separate lignin from cellulose through size reduction and differential solubility in the aqueous medium, eliminating the need for organic solvents and subsequent neutralization/filtering steps. The mechanical energy input directly achieves separation that would otherwise require multiple chemical processing stages.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This method achieves a high saccharification yield of 95%-98% with reduced costs and environmental impact, producing fermentable sugars efficiently and effectively for biofuel and biochemical production.

Implementation Method 1

homogenizing the first lignocellulose suspension obtained in the step (2) at a pressure of 50-100 atm

Methodology Applied
Scientific EffectHigh pressure: Pressurisation

Implementation Method 2

The homogenization is defined as follows: a premixing material enters a valve area at a low flow speed under high pressure, when the material enters a tiny gap between a controllable valve seat and a valve stem, the flow speed suddenly increases and can reach 300 m/second, and meanwhile, a huge pressure reduction is correspondingly generated. Thus, the strong void effect and vortex action are formed

Methodology Applied
Scientific EffectCavitation: Cavitation

Implementation Method 3

adding cellulase, β-glucosidase, and xylanase, and performing zymolysis of the second lignocellulose suspension for 36-72 hours

Methodology Applied
Scientific EffectEnzyme: Enzyme

Data Source

PatentEP2479281B1Process for treating lignocellulosic material
Publication Date: 2018.08.15 ANGEL YEAST CO LTD
  • EP2479281B1 patent drawingFigure 1
  • EP2479281B1 patent drawingFigure 2
  • EP2479281B1 patent drawingFigure 3

AI summary

A process for treating lignocellulosic material comprises the steps of: (1) crushing the lignocellulosic material; (2) mixing the resulting particles with water and dispersing the mixture by means of colloid mill to form a suspension; (3) high pressure homogenizing the suspension to obtain a particle with a particle diameter of 10-40µm; (4) buffering the suspension by using sodium acetate and acetic acid buffer solution, and then adding cellulase, β-glucosidase and xylanase and performing enzymolysis for 36-72 h.