Multilayer Limbal Cell Composition for Corneal Transparency
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Solution Overview
Problem
Current treatments for corneal blindness, such as keratoplasty, face challenges including insufficient donor tissue supply, rejection issues, and decreased graft survival, while epithelial stem cell transplantation alone fails to improve corneal clarity and vision due to neglecting the stromal component.
Innovation Solution
A pharmaceutical composition comprising a multilayer combination of limbal derived stromal cells and epithelial cells, with a customizable ratio, applied in a biomimetic approach to mimic natural corneal physiology, including a first layer of stromal cells with thrombin and a second layer of epithelial cells with fibrinogen, enhancing corneal clarity and visual recovery.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If keratoplasty is performed to replace damaged cornea, then corneal transparency is restored, but donor tissue supply is insufficient and rejection occurs
Solution Approach 1:
The invention extracts and isolates the essential functional components (epithelial stem cells and stromal stem cells) from donor corneal tissue, enabling these cells to be cultured, expanded, and transplanted separately. This eliminates the need for full corneal donation while preserving the regenerative capacity to restore corneal transparency.
Solution Approach 2:
The transplanted stem cells possess intrinsic self-renewal and differentiation capabilities that enable them to automatically regenerate the corneal epithelium and stroma without requiring continuous external intervention or additional donor tissue, thereby sustaining long-term corneal transparency.
2Stability of the object's composition
If epithelial stem cell transplantation is performed, then epithelial regeneration is achieved, but corneal clarity and visual recovery are not improved due to neglected stromal component
Solution Approach 1:
The invention merges epithelial stem cells and stromal stem cells into a combined cell composition that simultaneously addresses both the epithelial and stromal layers of the cornea. This dual-component approach ensures comprehensive regeneration of corneal structures, leading to both epithelial stability and improved corneal clarity for visual recovery.
Solution Approach 2:
The invention applies different cell types to different corneal layers: epithelial stem cells are directed to regenerate the epithelial layer while stromal stem cells are directed to regenerate the stromal layer. This localized cell differentiation ensures that each corneal layer receives the appropriate cellular components for optimal regeneration and transparency.
Data Source
AI summary
The present invention provides cell compositions, methods of production and its uses in corneal diseases. The invention discloses cell compositions and multilayer cell compositions comprising limbal epithelial cells and limbal stromal cells. The inventions are highly efficacious and represents an advancement over the existing therapeutic approaches in treatment or prevention of corneal diseases. The invention also discloses methods for preparing the compositions, methods of treatment and the uses of the composition in preventing and treating corneal diseases.