Line-Scan Structured Illumination for Motion-Tolerant Super-Resolution
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Solution Overview
Problem
Existing super-resolution imaging techniques require the target area to remain stationary, making them unsuitable for imaging live animals due to physiological movements, and they are limited by slow imaging speeds and the diffraction limit.
Innovation Solution
A super-resolution imaging method and apparatus based on line scan that uses multiple types of structured line excitation light with different illumination modes to illuminate a target area row-by-row, allowing for a single scan to achieve high resolution and tolerate movement during imaging.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional imaging techniques are used, then the imaging system is simple and easy to operate, but the imaging resolution is limited by the diffraction limit to around 250 nm
Solution Approach 1:
The imaging field is divided into multiple rows that are scanned sequentially. Each row is illuminated by structured line excitation light with specific patterns, and the scanning process segments the overall imaging into manageable row-by-row acquisitions, enabling super-resolution while maintaining system feasibility
Solution Approach 2:
Multiple types of structured line excitation light with different illumination modes are switched periodically during the row-by-row scanning process. This periodic switching of illumination patterns enables the accumulation of information needed for super-resolution reconstruction
2Speed
If existing super-resolution imaging techniques are used, then the imaging resolution exceeds the diffraction limit, but the imaging speed is slow and the target area must remain stationary
Solution Approach 1:
The structured line excitation light patterns are pre-designed and prepared before the actual imaging process. The illumination modes are configured in advance to match the row-by-row scanning sequence, allowing the system to tolerate movements up to 200 micrometers per second while maintaining super-resolution capability
Solution Approach 2:
The system transitions from static wide-field illumination to dynamic row-by-row scanning with switched structured line excitation light. This dynamic approach allows the imaging system to track and tolerate target area movements while maintaining high resolution, enabling imaging of live animals
3Productivity
If existing super-resolution imaging techniques are used, then the imaging resolution reaches 100 nm or smaller, but the imaging time is extended due to multiple scans and stationary requirement
Solution Approach 1:
The row-by-row scanning process continuously illuminates and detects each row sequentially without interruption. The structured line excitation light maintains continuous useful action during the scanning process, eliminating the need for repeated scans and extending the imaging time while preserving super-resolution quality
Data Source
AI summary
A super-resolution imaging method (100) and apparatus based on line scan. The super-resolution imaging method (100) based on line scan includes: providing multiple types of structured line excitation light, each of the multiple types of structured line excitation light having a different illumination mode (step S102); illuminating a target area in a manner of row-by-row scanning along a single first direction while switching the multiple types of structured line excitation light, such that each row in the target area is illuminated by a corresponding type of structured line excitation light among the multiple types of structured line excitation light (step S104); and detecting response light generated by the each row in the target area in response to being illuminated by the corresponding type of structured line excitation light (step S106).