Lipase Detergent Composition Compatible Surfactant System
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current detergent compositions face challenges in effectively removing lipid stains due to harsh environmental conditions, which hinder the functionality of lipases, necessitating the development of suitable lipases that can function optimally in such conditions.
Innovation Solution
A detergent composition comprising a mixture of linear alkylbenzenesulphonate, sodium laureth sulfate, and alcohol ethoxylates, combined with a polypeptide having at least 85% sequence identity to a specific mature polypeptide, enhancing cleaning activity at pH 8 and improving lipid stain removal.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If lipases are used in detergent compositions with multiple active ingredients, then lipid stain removal capability is improved, but the harsh environment created by other ingredients interferes with lipase functionality
Solution Approach 1:
The patent introduces a specific surfactant system (linear alkylbenzenesulphonate, sodium laureth sulfate, and alcohol ethoxylates) that acts as a compatible intermediary, allowing lipases to function effectively in detergent compositions. This surfactant system creates a microenvironment that supports lipase activity while maintaining the necessary cleaning properties of the detergent formulation.
Solution Approach 2:
The patent optimizes specific parameters including pH (maintaining pH 8 conditions where the lipase shows improved activity), surfactant concentrations (10-60 weight%), and lipase formulation to create conditions where lipase functionality is preserved despite the presence of multiple active ingredients. The specific lipase from Rhizopus microspores var. chinensis is engineered or selected to function optimally at pH 8.
2Productivity
If conventional detergent compositions are formulated with multiple active ingredients, then cleaning performance is enhanced, but the harsh environment hinders lipase ability to remove lipid stains
Solution Approach 1:
The surfactant system serves as a protective intermediary between the harsh detergent environment and the lipase enzyme. By selecting specific surfactants (linear alkylbenzenesulphonate, sodium laureth sulfate, and alcohol ethoxylates) that are compatible with lipase structure and function, the patent creates a formulation where the surfactants mediate the interaction between the harsh environment and the enzyme, reducing denaturation and maintaining catalytic activity.
Solution Approach 2:
The patent creates a composite detergent formulation where lipase is integrated with a specifically designed surfactant system. This composite approach allows the formulation to simultaneously provide the harsh cleaning environment needed for stain removal while protecting lipase functionality through the synergistic interaction between the surfactants and the enzyme.
3Productivity
If lipase activity is enhanced in harsh environments, then lipid stain removal is improved, but formulation complexity increases
Solution Approach 1:
The patent develops a lipase formulation that functions universally across standard detergent conditions (pH 8, presence of surfactants). The lipase from Rhizopus microspores var. chinensis is selected or engineered to have broad compatibility with common detergent ingredients, eliminating the need for highly specialized formulations and allowing the enzyme to function in conventional detergent environments.
Solution Approach 2:
The patent establishes specific parameter ranges (pH 8, 10-60 weight% surfactants including linear alkylbenzenesulphonate, sodium laureth sulfate, and alcohol ethoxylates) within which the lipase functions optimally. By defining these parameters, the patent simplifies formulation development while maintaining enhanced lipid stain removal capability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The composition demonstrates improved cleaning activity and lipid stain removal performance compared to compositions without surfactants, effectively addressing the harsh environmental limitations faced by lipases.
Implementation Method 1
Lipases have been employed in compositions for removal of lipid stains by hydrolyzing triglycerides to generate fatty acids
Implementation Method 2
the surfactant system comprises a mixture of linear alkylbenzenesulphonate (LAS), sodium laureth sulfate (SLES) and alcohol ethoxylates (AEO)
Data Source
AI summary
The present invention relates to a composition comprising: (a) at least one surfactant, at least one surfactant system, at least one soap, or any mixtures thereof; and (b) a polypeptide having lipase activity selected from the group consisting of: (I) a polypeptide having at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 2; (II) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, or (ii) the full-length complement of (i); (III) a polypeptide encoded by a polynucleotide having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1; (IV) a variant of the mature polypeptide of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more positions; and (V) a fragment of the polypeptide of (I), (II), (III), or (IV); wherein said composition is a cleaning and/or treatment composition. The invention furthermore relates to methods of producing and using the composition.