Dry Analytical Element for Pancreatic Lipase Using Alkylarylsulfonate Surfactants
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Solution Overview
Problem
Current dry analytical elements for pancreatic lipase analysis have low selectivity and multianalyte correlation, making them inadequate for reliable diagnosis of pancreatic diseases, and existing methods face challenges in maintaining substrate dispersion and particle size uniformity, leading to reduced reaction rates and increased environmental impact.
Innovation Solution
A dry analytical element comprising long-chain fatty acid glyceride, monoglyceride lipase, and a glycerine measurement reagent, with two or more anionic surfactants, specifically alkylarylsulfonates, to enhance pancreatic lipase selectivity and multianalyte correlation, using a water-impermeable support and layers of reagents and development materials like porous membranes and fabrics.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If long-chain fatty acid glyceride is used as substrate for pancreatic lipase measurement, then selectivity for pancreatic lipase is improved, but substrate dispersion and particle size uniformity deteriorate
Solution Approach 1:
The patent introduces a protective colloid as an intermediary substance to stabilize the dispersion of long-chain fatty acid glyceride substrate. The protective colloid prevents substrate aggregation and maintains uniform particle size distribution, resolving the contradiction between achieving high selectivity through long-chain substrate use and maintaining stable dispersion characteristics.
2Stability of the object's composition
If surfactant or protective colloid is used to disperse substrate, then substrate dispersion is improved, but environmental burden increases
Solution Approach 1:
The patent employs a protective colloid that can be easily degraded and has minimal environmental impact, replacing conventional surfactants that persist in the environment. This approach maintains effective substrate dispersion while significantly reducing environmental burden through the use of biodegradable materials.
3Stability of the object's composition
If triolein is emulsified and dispersed using ultrasound, then initial dispersion is improved, but aggregation occurs during drying and storage
Solution Approach 1:
The patent applies preliminary action by incorporating the protective colloid into the substrate dispersion process before drying and storage. This pre-protection prevents aggregation from occurring in the first place during subsequent processing and storage, rather than attempting to restore dispersion after aggregation has occurred.
Solution Approach 2:
The patent utilizes parameter changes by optimizing the concentration and type of protective colloid to maintain dispersion stability across varying conditions of drying and storage. By adjusting the protective colloid parameters, the system maintains stable dispersion without aggregation over extended periods.
4Stability of the object's composition
If decrease in oil-water interface surface area occurs, then substrate stability is improved, but reaction rate decreases
Solution Approach 1:
The patent employs parameter changes by optimizing the protective colloid concentration and substrate particle size distribution to maintain a large effective oil-water interface surface area while preventing aggregation. This allows the system to achieve both substrate stability and high reaction rate simultaneously through controlled parameter optimization.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution significantly improves the correlation coefficient of multianalyte correlation and lipase activity, providing higher specificity and accuracy in pancreatic lipase analysis without increasing colipase amounts, thus enhancing diagnostic reliability and reducing environmental impact.
Implementation Method 1
the development layer and/or the reagent layer comprise two or more anionic surfactants wherein at least one of the anionic surfactants is an alkylarylsulfonate
Implementation Method 2
monoglyceride lipase, and a glycerine measurement reagent
Implementation Method 3
using a water-impermeable support and layers of reagents and development materials like porous membranes and fabrics
Data Source
AI summary
It is an object of the present invention to provide a dry analytical element for pancreatic lipase analysis having high selectivity with respect to pancreatic lipase, whose multianalyte correlation has been improved. The present invention provides a dry analytical element for measurement of pancreatic lipase contained in a body fluid, which comprises at least one development layer and/or reagent layer containing diglyceride or triglyceride of long-chain alkyl fatty acid having 12 to 22 carbon atoms, monoglyceride lipase, and a glycerine measurement reagent, wherein the development layer and/or the reagent layer comprise two or more types of anionic surfactants and at least one type of the anionic surfactant is alkylarylsulfonate.