Lipase Sequence Variants for Low-Odor Lipid Stain Hydrolysis
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Solution Overview
Problem
Lipolytic enzymes used for lipid stain removal generate unpleasant odors during the wash process, which are often masked with perfumes but not effectively reduced.
Innovation Solution
Development of lipase variants with specific substitutions at certain positions, achieving at least 60% to less than 100% sequence identity to a parent lipase, which reduce odor generation during lipid stain removal.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If lipolytic enzymes are used for lipid stain removal, then cleaning effectiveness is improved, but unpleasant odor is generated
Solution Approach 1:
The patent applies parameter changes by modifying the amino acid sequence of the lipase enzyme through specific substitutions at defined positions. These molecular-level parameter changes alter the enzyme's catalytic properties to reduce free fatty acid release while maintaining cleaning effectiveness, directly resolving the contradiction between productivity and harmful odor generation
Solution Approach 2:
The patent converts the harmful odor-generating activity of lipase into a beneficial property by engineering variants that selectively reduce unwanted free fatty acid production. The modified enzymes maintain their lipid-stain removal capability while the harmful odor-generating side effect is transformed into a controlled feature, achieving both cleaning effectiveness and odor reduction
2Object-affected harmful factors
If perfume is added to mask the odor, then odor perception is reduced, but the underlying problem of odor generation remains unsolved
Solution Approach 1:
The patent extracts and eliminates the root cause of odor generation by modifying the lipase enzyme itself to produce fewer free fatty acids. Instead of merely masking the odor with perfume, the invention removes the source of the problem at the molecular level, providing a fundamental solution rather than a superficial掩盖
Solution Approach 2:
Rather than adding substances to mask the odor (conventional approach), the patent inverts the strategy by modifying the enzyme to reduce odor generation at its source. This inverse approach addresses the problem fundamentally by changing the enzyme's properties rather than counteracting its effects
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The lipase variants effectively minimize odor generation compared to using parent lipases, while maintaining significant lipase activity.
Implementation Method 1
Lipases hydrolyze a broad spectrum of ester bonds some of which result in release of free fatty acids that generate odor
Data Source
AI summary
The present invention related to lipase variants having lipase activity and having between 60% to less than 100% sequence identity to a parent lipase or a fragment thereof, wherein the variant comprises one or more substitutions selected from H198A/D/E/F/G/I/L/N/Q/S/T/V/Y, F7H/K/R, F51A/I/L/V/Y, T143A/G/SN, A150G/V, N200H/K/Q/R, I202G/L/V, S224C/F/H/I/L/P/Y, L227D/E/K/R, V228P, P229H/K/R, V230H/K/L/R, I255A/G/N/P/S/T/V/Y, P256A/K/N/Q/R/S/T/W, A257F/H/I/L/V/Y, L259F/Y, and W260D/E/F/H/I/L/N/Q/S/T/Y using SEQ ID NO:10 for position numbering or selected from H198A/D/E/F/G/I/L/N/Q/S/T/V/Y, F7H/K/R, F51A/I/L/V/Y, T143A/G/S/V, A150G/V, N200H/K/Q/R, I202G/L/V, S224C/F/H/I/L/P/Y, L227D/E/K/R, V228P, P229H/K/R, V230H/K/L/R, I255A/G/N/P/S/T/V/Y, T256A/K/N/Q/R/S/P/W, A257F/H/I/LN/Y, L259F/Y, and W260D/E/F/H/I/L/N/Q/S/T/Y using SEQ ID NO:2 for position numbering. Further the invention relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; to compositions comprising the variants as well as methods of producing and using the variants.