Lipase Variants for Stable Detergent Enzyme Compositions
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Solution Overview
Problem
Current detergent, cleaning, and fabric care compositions face challenges with lipases due to interference from active ingredients, leading to stability issues during storage and harsh environmental conditions.
Innovation Solution
Development of lipase variants with specific substitutions at positions corresponding to T231R+N233R and additional positions such as D96E, D111A, D254S, G163K, P256T, G91T, and G38A, enhancing stability and activity in detergent compositions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If lipases are used in detergent compositions, then lipid stain removal capability is improved, but stability under storage conditions and in harsh environmental conditions deteriorates
Solution Approach 1:
The patent applies parameter changes by modifying the amino acid sequence of the lipase through specific substitutions (T231R, N233R, and combinations with D96E, D111A, G163K, etc.). These parameter changes in the protein structure enhance the enzyme's stability under storage conditions and in harsh detergent environments while preserving its lipolytic activity for effective lipid stain removal.
2Productivity
If lipases are used in detergent compositions, then lipid stain removal capability is improved, but stability in the presence of proteases and surfactants deteriorates
Solution Approach 1:
The patent modifies the lipase's amino acid parameters through specific substitutions including T231R, N233R, and various combinations with D96E, D111A, G163K, P256T, G91T, and G38A. These parameter changes confer resistance to proteolytic degradation and maintain structural integrity in the presence of surfactants, thereby improving reliability while preserving productivity.
3Productivity
If lipases are used in detergent compositions, then lipid stain removal capability is improved, but chemical stability and oxidation stability deteriorates
Solution Approach 1:
The patent employs parameter changes in the form of specific amino acid substitutions (T231R, N233R, and combinations with D96E, D111A, G163K, etc.) that enhance the lipase's resistance to chemical degradation and oxidation. These modifications strengthen the protein's chemical stability while maintaining its catalytic function for effective lipid stain removal.
4Productivity
If lipases are used in detergent compositions, then lipid stain removal capability is improved, but thermostability deteriorates
Solution Approach 1:
The patent applies parameter changes through specific amino acid substitutions including T231R, N233R, and various combinations with D96E, D111A, G163K, P256T, G91T, and G38A. These parameter modifications enhance the lipase's thermostability by strengthening intramolecular interactions and improving structural rigidity, allowing the enzyme to maintain activity at higher temperatures typical of washing conditions.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The lipase variants exhibit improved stability under storage conditions, in the presence of proteases and surfactants, chemical stability, oxidation stability, and thermostability, maintaining effective performance in detergent compositions.
Implementation Method 1
Lipases have been employed in compositions for the removal of lipid stains by hydrolyzing triglycerides to generate fatty acids
Data Source
AI summary
The present invention relates to lipase variants and detergent compositions comprising a variant of a parent lipase which variant has lipase activity, has at least 60% but less than 100% sequence identity with SEQ ID NO: 2, and comprises substitutions at positions corresponding to T231R+N233R and at least one or more (e.g., several) of D96E, D111A, D254S, G163K, P256T, G91T, and G38A of SEQ ID NO: 2. The present invention also relates to use of the compositions, methods of producing the compositions, and methods of cleaning. The present invention furthermore relates to variants and polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides.