Lipid Pre-Formulation for Sustained Peptide Release

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Solution Overview

Problem

Current controlled-release systems for bioactive agents, particularly peptides and proteins, face challenges due to poor bioavailability and rapid degradation, leading to inconvenient administration routes and irritation issues, with polymer-based depot compositions causing burst release profiles and irritation.

Innovation Solution

A lipid-based pre-formulation comprising a sorbitan ester, phospholipid, and a biocompatible organic solvent, eliminating the need for a liquid crystal hardener, which forms a non-lamellar liquid crystalline phase upon contact with aqueous fluids, providing a controlled release matrix with a non-burst release profile and improved bioavailability.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Duration of action of stationary object

If polymer-based depot compositions are used for controlled release, then sustained release over long period is achieved, but burst release profile and irritation at injection site occur

Engineering Contradiction:
Improveduration of controlled releaseVSAvoidirritation and burst release
Core Design Contradiction:
Duration of action of stationary objectVSObject-generated harmful factors

Solution Approach 1:

The patent changes the fundamental parameter of the release matrix from polymer-based to lipid-based pre-formulation. The lipid components (sorbitan ester, phospholipid, and biocompatible organic solvent) undergo phase transition upon contact with aqueous media to form a controlled release matrix, eliminating the burst release and irritation issues associated with polymer degradation products like acetic acid, lactic acid, and glycolic acid.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The lipid-based pre-formulation exploits phase transition phenomena where the lipid components spontaneously reorganize from an isotropic liquid state into a structured controlled release matrix (such as liquid crystalline phases or gel structures) upon contact with body fluids. This phase transition enables sustained release without the harmful effects of polymer degradation, providing a non-burst release profile while maintaining biocompatibility.

Inventive Principle:
Principle #36Phase transitions

2Reliability

If peptides and proteins are administered to achieve therapeutic effect, then bioactivity is achieved, but rapid degradation by proteolytic enzymes reduces bioavailability

Engineering Contradiction:
Improvebioactivity of peptideVSAvoiddegradation of peptide
Core Design Contradiction:
ReliabilityVSLoss of substance

Solution Approach 1:

The lipid-based pre-formulation acts as a protective shell or matrix that encapsulates the peptide or protein bioactive agent. This lipid matrix protects the peptide from exposure to proteolytic enzymes in the body, thereby preventing enzymatic degradation and improving bioavailability. The flexible lipid structure allows for controlled release of the protected peptide over time.

Inventive Principle:
Principle #30Flexible shells and thin films

3Reliability

If high concentration of bioactive agent is administered to overcome rapid clearance, then therapeutic level is maintained, but frequency of administration increases

Engineering Contradiction:
Improvetherapeutic concentrationVSAvoidfrequency of dosing
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The lipid-based pre-formulation enables a single administration to provide sufficient bioactive agent at therapeutic levels over an extended period. The controlled release matrix releases the bioactive agent gradually, maintaining therapeutic concentrations without requiring frequent dosing, thus reducing the time loss associated with repeated administrations.

Inventive Principle:
Principle #16Partial or excessive action

4Reliability

If non-oral routes of administration are used for peptides, then bioavailability is improved, but self-administration becomes difficult and compliance decreases

Engineering Contradiction:
Improvebioavailability of peptideVSAvoidself-administration convenience
Core Design Contradiction:
ReliabilityVSEase of operation

Solution Approach 1:

The lipid-based pre-formulation changes the physical parameters of the formulation to enable easy self-administration. The pre-formulation has properties that allow it to be administered through smaller needles via subcutaneous injection, making self-administration feasible and improving patient compliance while maintaining the bioavailability benefits of non-oral routes.

Inventive Principle:
Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The formulation allows for a controlled and sustained release of bioactive agents, reducing irritation and improving bioavailability, enabling self-administration through smaller needles and maintaining therapeutic levels without the need for frequent dosing.

Implementation Method 1

comprising lipids that upon exposure to water or aqueous media, such as body fluids, spontaneously undergo at least one phase transition, thereby forming a controlled release matrix

Methodology Applied
Scientific EffectPhase transition: Phase Change

Implementation Method 2

forms a non-lamellar liquid crystalline phase upon contact with aqueous fluids

Methodology Applied
Scientific EffectLiquid crystalline phase formation: Liquid Crystals

Data Source

PatentEP3236937B1Controlled-release formulations
Publication Date: 2022.05.04 CAMURUS AB
  • EP3236937B1 patent drawingFigure 1
  • EP3236937B1 patent drawingFigure 2
  • EP3236937B1 patent drawingFigure 3

AI summary

The present invention relates to pre-formulations comprising low viscosity, non- liquid crystalline, mixtures of: a) at least one ester of a sugar or sugar derivative; b) at least one phospholipid; c) at least one biocompatible, oxygen containing, low viscosity organic solvent; wherein the pre-formulation forms, or is capable of forming, at least one liquid crystalline phase structure upon contact with an aqueous fluid; with the proviso that the pre-formulation does not further comprise a liquid crystal hardener. The preformulations are suitable for generating parenteral, non-parenteral and topical depot compositions for sustained release of active agents. The invention additionally relates to a method of delivery of an active agent comprising administration of a preformulation of the invention, a depot composition formed by exposing pre-formulations of the invention to an aqueous fluid, a method of treatment comprising administration of a preformulation of the invention and the use of a preformulation of the invention.