Lipoprotein Analysis Calculation Method
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Conventional methods for independently measuring cholesterol ester and free cholesterol concentrations in lipoproteins are time-consuming and costly, and existing lipoprotein classification methods lack a theoretical foundation, leading to confusion in defining lipoprotein subclasses and comparing samples across different gel filtration columns.
Innovation Solution
A method that calculates the concentration of cholesterol ester or free cholesterol using the concentrations of total cholesterol and triglyceride, and the size of lipoprotein particles, employing modeled relationships expressed through specific formulae and an oil drop model to determine lipoprotein particle concentration, allowing for accurate analysis regardless of column specifications.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional methods are used to independently measure cholesterol ester and free cholesterol concentrations, then measurement accuracy is maintained, but measurement time increases and cost increases
Solution Approach 1:
The invention creates a mathematical model that copies the relationship between total cholesterol, triglyceride, and cholesterol ester concentrations established through conventional measurement methods. This model allows calculation of cholesterol ester and free cholesterol concentrations from routinely measured parameters, eliminating the need for time-consuming independent measurements while maintaining accuracy through validated mathematical relationships
Solution Approach 2:
The invention replaces the mechanical/chemical measurement system with a computational system. Instead of using complex chemical assays to independently measure cholesterol ester and free cholesterol, the system uses mathematical calculations based on routinely measured total cholesterol and triglyceride values, substituting physical measurement with computational derivation
2Measurement precision
If gel filtration chromatography is used to classify lipoproteins, then lipoprotein fractions are obtained, but particle size definitions vary across different columns causing confusion
Solution Approach 1:
The invention changes the parameter used for lipoprotein classification from absolute particle size (which varies by column) to relative elution position or retention time. By establishing reference peak positions from metabolic disorder patients with narrow size ranges, the system creates a standardized classification framework that works across different gel filtration columns, transforming an absolute measurement problem into a relative positioning solution
Solution Approach 2:
The invention creates a universal classification method that functions across multiple different gel filtration column types. By using relative elution positions and reference peak positions rather than column-specific particle size calibrations, the same classification approach can be applied universally to different columns, making the system adaptable and versatile across various laboratory configurations
3Measurement precision
If 20 subclasses of lipoproteins are classified, then detailed lipoprotein profile is obtained, but the method can only be applied to specific columns without theoretical foundation
Solution Approach 1:
The invention transforms the classification system from being based on absolute particle size parameters to being based on relative elution behavior. By using retention times and peak positions relative to reference standards, the detailed 20-subclass classification can be applied to any gel filtration column, removing the restriction to specific columns while maintaining the theoretical foundation through reference-based positioning
Data Source
Figure 1
Figure 2
Figure 3a~3d
AI summary
According to the present invention, the concentration of cholesterol ester or free cholesterol can be calculated using the concentrations of total cholesterol and triglyceride in lipoproteins contained in a sample. Further, according to the present invention, the concentration of lipoprotein particles in a fraction can be calculated using the lipoprotein particle size in the fraction. In the present invention, the concentrations of total cholesterol (Cho) and triglyceride (TG) contained in a subject sample are detected. Using the concentrations of Cho and TG thus detected, the concentration of cholesterol ester (CE) or free cholesterol (FC) is calculated. Further, using the concentration of cholesterol ester (CE) or free cholesterol (FC) thus calculated, the concentration of lipoprotein particles in a fraction can be calculated.