Liquid Biopsy Sample Fractionation for Stable Multi-Component Analysis
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Solution Overview
Problem
Existing methods for preparing biological samples, particularly for liquid biopsy, face challenges such as sample degradation, interference from preservatives, cellular loss during processing, and the need for multiple collections and analyses, leading to unreliable results and increased costs.
Innovation Solution
An apparatus for automated sample preparation that separates and stabilizes different components of a biological sample, including immobilized cellular, suspension cellular, and liquid fractions, using a box-like structure with centrifugation and controlled dispensing, allowing simultaneous processing of multiple samples without manual intervention.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If manual sample preparation methods are used, then flexibility and adaptability are maintained, but labor intensity is high and throughput is limited
Solution Approach 1:
The sample preparation system is divided into distinct functional modules: a first processing unit for initial sample treatment, a second processing unit for further processing, and a third processing unit for final preparation. Each module performs specific operations, enabling automated high-throughput processing while maintaining flexibility through modular configuration.
Solution Approach 2:
The apparatus is designed with universal processing units that can handle multiple sample types and preparation protocols. The system incorporates adjustable parameters and configurable processing steps that allow the same automated apparatus to perform various sample preparation tasks, reducing the need for multiple specialized devices.
2Loss of time
If conventional sample preparation procedures are used, then established protocols are maintained, but time-consuming steps and manual intervention are required
Solution Approach 1:
The system incorporates pre-programmed processing steps and pre-configured parameters that automatically execute established protocols. Sample preparation steps are predetermined and automatically performed in sequence, eliminating manual intervention while maintaining protocol consistency. The apparatus can be pre-loaded with standard protocols that ensure reliable, repeatable results.
Solution Approach 2:
The apparatus incorporates monitoring and feedback mechanisms that track sample processing progress and quality parameters. The system can detect when specific steps are completed successfully and automatically adjust subsequent operations, ensuring consistent protocol execution while reducing overall processing time through real-time optimization.
3Productivity
If automated processing is implemented, then throughput increases, but cost and complexity of the system increase
Solution Approach 1:
The system employs a nested modular architecture where smaller processing units are contained within a larger framework. The first, second, and third processing units are organized in a hierarchical manner, with each unit containing specialized components that can be independently manufactured and assembled. This nesting approach reduces overall system complexity and manufacturing cost while maintaining high throughput capability.
Solution Approach 2:
The apparatus incorporates waste management features that automatically discard spent consumables and recover valuable materials. The system includes mechanisms for collecting and disposing of waste samples and for recovering and recycling valuable reagents, reducing operational costs and environmental impact while maintaining automated high-throughput processing.
Data Source
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AI summary
The present invention relates to an apparatus for the automated processing of a body fluid sample, preferably for analysis of liquid biopsy, configured to obtain one or more informative components of the fluid sample separate and distinct from each other, wherein a first component comprises cellular elements which result to be immobilized on planar supports, wherein a second component comprises suspension sample cells, and wherein a third component comprises the sample liquid fraction and it is preferably contained in a test tube. The invention further relates to a method for preparation of said components implemented by said apparatus.