Liquid Chromatography Correction for Ghost Peaks
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Solution Overview
Problem
Current liquid chromatography devices fail to account for room temperature fluctuations and 'ghost peaks' during sample measurement, leading to baseline drift and erroneous component collection in preparative liquid chromatography.
Innovation Solution
A liquid chromatograph analyzing device with a correction parameter holding section and a correction formula setting unit that allows for real-time chromatogram correction using temperature data and background information, eliminating baseline drift and ghost peaks by successively creating chromatograms that reflect environmental conditions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If background data subtraction is performed after sample measurement, then baseline drift effects are corrected, but ghost peaks cannot be eliminated in real-time and complex pre-processing is required
Solution Approach 1:
The patent measures and stores background data (including ghost peaks) in advance before sample measurement. This preliminary action allows the system to have correction data ready, eliminating the need for complex real-time forecasting and enabling straightforward subtraction of background effects during sample analysis.
Solution Approach 2:
The system uses the measured background data as feedback to correct sample chromatograms in real-time. By continuously comparing sample data against the stored background data and applying corrections, the system automatically eliminates ghost peaks and baseline drift without requiring manual intervention or complex pre-processing setup.
2Reliability
If real-time chromatogram correction is implemented, then ghost peaks are eliminated during component collection, but additional correction parameters and processing are required
Solution Approach 1:
Background data including ghost peak information is measured and stored in advance. This preliminary measurement provides the correction parameters needed for real-time processing, allowing the system to reliably identify and exclude ghost peaks during component collection without adding complex real-time measurement systems.
3Manufacturing precision
If temperature gradient method is used for separation, then analytical separation is improved, but baseline drift occurs due to temperature fluctuations
Solution Approach 1:
The system uses temperature sensors to continuously monitor environmental temperature changes and feeds this information back to the correction system. By comparing actual temperature data against reference conditions and applying corrections based on stored background data, the system compensates for temperature-induced baseline drift while maintaining the analytical benefits of temperature gradient separation.
Solution Approach 2:
The patent introduces background data as an intermediary element that mediates between the temperature gradient separation process and the final chromatogram. This background data acts as a reference model that captures temperature effects, allowing the system to subtract these effects and stabilize the baseline without interfering with the separation process itself.
Data Source
AI summary
A liquid chromatograph analyzing device, which without an analyst having to set complicated processing conditions before measuring a sample, is able to processes unaffected by fluctuations in room temperature and appearance of ghost peaks. In a liquid chromatograph analyzing device, an automatic sampler, a liquid sending pump, a column oven and a detection device are controlled by a calculation processing device. In addition to chromatograph creation unit, which creates chromatograms of a sample based on input detection signals, the calculation processing device has a correction parameter holding section and correction formula setting unit. The correction formula setting unit provides to the liquid chromatograph analyzing device a functionality for the analyst to set correction formulas for incorporating in the chromatograms created by the chromatograph creation unit changes to environmental conditions such as fluctuations in temperature around the liquid chromatograph analyzing device.