Liquid composition for molecular diagnostics by PCR
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Solution Overview
Problem
Current methods for extracting nucleic acids from pathogens require additional purification steps to remove inhibitory chemicals, leading to increased time, cost, and risk of false negative results due to the use of ionic detergents and other substances that interfere with PCR reactions.
Innovation Solution
A liquid composition containing 0.01-0.1 vol.% ionic detergent, 1-4 vol.% non-ionic detergent, and Na3PO4/NaHCO3, which effectively disrupts viral capsids and cell membranes, allowing direct use in PCR without the need for extensive purification, thereby reducing inhibitory effects and enhancing nucleic acid extraction efficiency.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If ionic detergents are used to disrupt viral capsids and cell membranes, then nucleic acid extraction efficiency is improved, but inhibitory effects on PCR reactions occur
Solution Approach 1:
The patent removes ionic detergents from the liquid composition to eliminate their harmful inhibitory effects on PCR reactions. By extracting the problematic component while retaining effective non-ionic detergents, the solution achieves both efficient nucleic acid release and PCR compatibility.
Solution Approach 2:
The patent converts the harmful effect of ionic detergents into a beneficial approach by using only non-ionic detergents, which provide the necessary disruption of viral capsids and cell membranes without the side effect of PCR inhibition. This transforms a harmful substance-based solution into a safe one.
2Reliability
If additional purification steps are performed to remove inhibitory chemicals, then PCR inhibition is reduced, but processing time and cost increase
Solution Approach 1:
The patent performs preliminary action by designing a liquid composition that prevents the formation of harmful substances in the first place. By using non-ionic detergents from the outset, the composition avoids generating PCR-inhibitory chemicals, eliminating the need for subsequent purification steps and saving time.
Solution Approach 2:
The liquid composition is designed to be self-sufficient, simultaneously achieving nucleic acid extraction and ensuring PCR compatibility without requiring external purification interventions. The composition itself provides all necessary functions, making the process autonomous and efficient.
3Manufacturing precision
If isolation kits are used for RNA/DNA purification, then nucleic acid quality is improved, but costs and complexity increase
Solution Approach 1:
The patent extracts and eliminates the need for complex isolation kits by using a simplified liquid composition that directly provides both extraction and PCR-compatible conditions. This removes the requirement for additional purification devices and procedures.
Solution Approach 2:
The liquid composition performs multiple functions simultaneously: it disrupts viral capsids and cell membranes, releases nucleic acids, and ensures PCR compatibility. This multi-functionality replaces the need for separate isolation and purification steps required by traditional kits.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The composition enables efficient extraction and storage of nucleic acids for several days at normal temperatures, reducing laboratory complexity, cost, and the risk of false negatives, while maintaining PCR sensitivity and robustness.
Implementation Method 1
The liquid composition contains 0.01-0.1 vol.% of at least one ionic detergent, 1-4 vol.% of at least one non-ionic detergent
Implementation Method 2
It has been proposed that non-ionic detergent may enhance the effect of ionic detergents in RNA/DNA extraction
Implementation Method 3
The liquid composition contains Na3PO4 and/or NaHCO3
Data Source
AI summary
The present invention provides a liquid composition for extracting nucleic acid(s) from pathogens, said liquid composition containing 0.01-0.1 vol. % of at least one ionic detergent, 1-4 vol. % of at least one non-ionic detergent, and Na3PO4 and/or NaHCO3. Furthermore, methods of using this liquid composition are provided.