Liquid Detergent Protease Stabilization with Peptidic Inhibitors
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Solution Overview
Problem
Existing washing and cleaning agents face challenges in maintaining the stability and activity of proteases due to auto-proteolysis during storage and use, particularly in liquid formulations, leading to reduced cleaning performance.
Innovation Solution
Incorporating specific peptidic inhibitors that stabilize proteases by forming a reversible complex, such as those derived from Bacillus pumilus with defined amino acid substitutions, in combination with other enzymes to enhance stability and activity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If proteases are used in liquid washing and cleaning agents, then cleaning performance is improved, but protease stability during storage deteriorates due to auto-proteolysis
Solution Approach 1:
A peptidic inhibitor is introduced as an intermediary substance that temporarily binds to the protease, preventing auto-proteolysis during storage. The inhibitor acts as a mediator that protects the protease from degrading itself, allowing the protease to remain stable in liquid formulations without compromising its cleaning function when activated.
Solution Approach 2:
The invention changes the chemical parameter of the protease by introducing specific amino acid substitutions (e.g., positions 9, 130, 133, 144, 217, 252, 271) that modify its stability characteristics. These parameter changes in the protease structure enable it to resist auto-proteolysis while maintaining catalytic activity, resolving the contradiction between stability and performance.
2Productivity
If proteases are used in water-containing formulations, then cleaning effectiveness is enhanced, but protease degradation accelerates
Solution Approach 1:
The peptidic inhibitor serves as a protective intermediary that allows the protease to function effectively in water-containing formulations during the cleaning process while preventing degradation during storage. The inhibitor can be activated or removed during the wash cycle, enabling the protease to operate in aqueous environments without self-destruction.
Solution Approach 2:
The protease is pre-modified through specific amino acid substitutions before being formulated into the liquid agent. This preliminary structural modification prepares the protease to resist water-induced degradation and auto-proteolysis, extending its functional duration in aqueous formulations without sacrificing cleaning effectiveness.
3Reliability
If reversible protease inhibitors are added to stabilize proteases, then protease stability improves, but cleaning performance may be reduced due to inhibited activity
Solution Approach 1:
The peptidic inhibitor is designed with specific peptide sequences and lengths (50-200 amino acids) that optimize its binding characteristics. The inhibitor's parameters are tuned to provide strong stabilization during storage while allowing sufficient protease activity during cleaning, resolving the contradiction between stability and performance through precise parameter control.
Solution Approach 2:
The inhibitor-protease complex exhibits dynamic behavior where the inhibitor provides strong binding during storage conditions but allows protease activation during cleaning. The system transitions from a stabilized inactive state during storage to an active cleaning state when conditions change, maintaining both stability and performance at different stages.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The peptidic inhibitors significantly improve the storage stability and residual activity of proteases, maintaining effective cleaning performance over time, especially in liquid agents.
Implementation Method 1
Incorporating specific peptidic inhibitors that stabilize proteases by forming a reversible complex
Implementation Method 2
The peptidic inhibitors significantly improve the storage stability and residual activity of proteases, maintaining effective cleaning performance over time
Data Source
AI summary
The invention relates to washing and cleaning agents, in particular, liquid washing and cleaning agents, and particularly preferably liquid textile washing agents, comprising a) at least one protease, preferably in an amount of 0.0001 to 1 wt. %, relative to the total weight of the washing and cleaning agent, b) at least one peptide (peptidic inhibitor), preferably in a 0.5-, 1.0-, 1.5-, 2.0-, 2.5-, 3.0-, 3.5-, 4.0-, 4.5-, 5.0-, 10-, 15-, 18-, 20-, 25-, 27-, or 30-fold molar excess relative to the molarity of the protease used, preferably in a 15-, 18-, 20-, 25-, or 27-fold molar excess, the peptide preferably having a length of 50 to 200, preferably 55 to 190, and more preferably 60 to 180, amino acid functional groups, and c) at least one washing and cleaning agent ingredient, preferably in an amount of 0.01 to 99.9 wt. %, relative to the total weight of the washing and cleaning agent. Also included in the invention are the corresponding washing and cleaning processes, the use of the agents described herein, and the use of a peptidic inhibitor to improve the stability of a protease in washing or cleaning agents.