Liquid Mycorrhiza Composition Stability via Bactericide Mediation

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Solution Overview

Problem

Current fertilizer blends containing mycorrhizas face stability issues in liquid form, limiting their effectiveness in plant colonization, and existing technologies have not successfully developed stable liquid mycorrhiza compositions for improved colonization efficiency.

Innovation Solution

A liquid mycorrhiza composition comprising 0.2-5% mycorrhizas and 50-3,000 ppm bactericide or bacteriostat, specifically using strains like Glomus and Laccaria, combined with bactericides such as Kathon and Bronopol, to enhance mycorrhizal colonization of plant roots.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If mycorrhizas are added to liquid fertilizers, then colonization efficiency is improved, but stability deteriorates

Engineering Contradiction:
Improvecolonization efficiencyVSAvoidliquid composition stability
Core Design Contradiction:
ProductivityVSStability of the object's composition

Solution Approach 1:

A bactericide or bacteriostat is introduced as an intermediary substance to mediate between the mycorrhizas and the liquid fertilizer environment. The bactericide protects the mycorrhizal spores from bacterial contamination and degradation, enabling them to remain stable in liquid form while maintaining their colonization capability. This intermediary agent resolves the contradiction by creating a protective environment that allows both liquid stability and biological activity to coexist.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The invention creates a composite liquid composition that integrates multiple components: liquid fertilizer, mycorrhizal spores, and bactericide/bacteriostat. This composite formulation combines substances with different properties to achieve a synergistic effect where the bactericide protects the mycorrhizas while the liquid medium provides ease of application. The composite structure allows the system to simultaneously achieve stability (through the protective bactericide) and colonization efficiency (through the viable mycorrhizal spores in liquid form).

Inventive Principle:
Principle #40Composite materials

2Stability of the object's composition

If bactericides are used at high rates to eliminate bacterial contamination, then mycorrhizal stability is improved, but harmful effects on mycorrhizas increase

Engineering Contradiction:
Improvemycorrhizal stabilityVSAvoidharmful effects on mycorrhizas
Core Design Contradiction:
Stability of the object's compositionVSObject-generated harmful factors

Solution Approach 1:

The invention optimizes the concentration parameters of bactericides and bacteriostats to achieve effective bacterial control while minimizing harm to mycorrhizas. By carefully adjusting the dosage levels and selecting appropriate substances from the specified groups, the formulation achieves a parameter balance where bacterial contamination is suppressed but mycorrhizal viability is preserved. This parameter optimization resolves the contradiction between stability and harmful effects.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The use of bactericides and bacteriostats as temporary protective agents that function during the critical period of liquid formulation storage and application. These substances provide short-term protection against bacterial contamination during the period when mycorrhizas are most vulnerable in liquid form, after which their protective function diminishes. This approach allows effective stabilization without requiring permanent or excessively strong chemical interventions that would harm the mycorrhizas.

Inventive Principle:
Principle #27Cheap short-living objects (Disposable)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The composition significantly increases mycorrhizal colonization, leading to improved plant growth and yield, with treated plants showing higher mycorrhizal infectivity and colonization rates compared to untreated controls.

Implementation Method 1

bactericides are commonly used to eliminate unwanted bacterial contamination. The preservatives used at low rates are designed to kill vegetative bacteria while leaving spores unharmed.

Methodology Applied
Scientific EffectAntimicrobial action: Preservative

Implementation Method 2

The functional symbiosis provides a suitable and sufficient carbohydrate source for the fungal symbiont. The plant symbiont benefits can be numerous and include improved nutrient and water uptake, additional carbon acquisition, increased sink strength for photosynthate translocation

Methodology Applied
Scientific EffectMycorrhizal colonization:

Data Source

PatentUS8883679B2Liquid mycorrhiza compositions
Publication Date: 2014.11.11 NOVOZYMES AS

AI summary

The present invention relates to liquid mycorrhiza compositions and to methods for colonizing a plant, grass, tree or shrub with one or more mycorrhizas.