Liquid-State Pathological Control Product for Antigen Stability
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Solution Overview
Problem
Current methods for establishing effective positive and negative controls in situ section staining technologies, such as immunohistochemistry and in situ hybridization, are labor-intensive, time-consuming, and result in tissue loss, making them impractical for widespread use, especially due to the oxidation of paraffin sections which leads to antigen loss.
Innovation Solution
A method for preparing a liquid-state dripping or coating pathological quality control product by creating a suspension or homogenate of tissue- or cell-embedded sections, using ethanol preservation to maintain antigen stability and simplicity in preparation, allowing for convenient and reliable use as positive or negative controls.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If gene chips or sausage-shaped paraffin sections are used as controls, then control accuracy can be maintained, but the workload increases, time consumption increases, and tissue loss occurs
Solution Approach 1:
The patent creates liquid-state control products that are copies of the antigen expression patterns found in original tissue sections. By preparing suspensions or homogenates from embedded sections with known antigen expression, the invention reproduces the essential control features without requiring physical paraffin sections, thereby reducing workload while maintaining control accuracy.
Solution Approach 2:
The patent changes the physical state parameter of the control material from solid paraffin sections to liquid suspension or homogenate form. This parameter change enables the control material to be applied directly to slides without complex sectioning procedures, significantly reducing preparation time and workload while preserving the antigen expression characteristics needed for accurate control.
2Reliability
If gene chips or sausage-shaped paraffin sections are used as controls, then control functionality is achieved, but time consumption increases and preparation steps become complicated
Solution Approach 1:
The patent performs preliminary preparation by embedding tissue sections and preserving them with ethanol to maintain antigen expression before they are needed. The suspensions or homogenates are prepared in advance and can be stored, eliminating the need for time-consuming sectioning and mounting procedures at the time of use, thus reducing preparation time while maintaining control functionality.
Solution Approach 2:
By creating liquid-state copies of the control material that capture the essential antigen expression patterns, the patent eliminates the need for complex paraffin sectioning and mounting steps. These liquid copies can be directly applied to slides, significantly simplifying the preparation process and reducing time consumption while preserving control functionality.
3Stability of the object's composition
If paraffin sections are used as controls, then control structure is maintained, but oxidation occurs leading to antigen loss
Solution Approach 1:
The patent converts the potential harm of oxidation by using ethanol preservation instead of paraffin embedding. Ethanol acts as a preservative that prevents oxidation while maintaining antigen integrity. The liquid-state control materials are prepared in a way that eliminates the oxidation problem inherent in paraffin sections, thus protecting antigen integrity while maintaining structural information needed for control functionality.
Solution Approach 2:
The patent changes the preservation parameter from paraffin embedding to ethanol preservation. This parameter change eliminates the oxidation issue associated with paraffin sections while maintaining the structural and antigenic properties needed for effective control. The liquid-state format combined with ethanol preservation creates a stable system that protects antigen integrity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method ensures accurate and reliable pathological detection results by maintaining antigen expression and structure, reducing workload, and enabling mass production and commercialization of control sections, thus improving the accuracy and efficiency of laboratory tests.
Implementation Method 1
adding an ethanol solution (preferably 75% ethanol solution) to the processed control for preserving for standby use
Implementation Method 2
adding dimethylbenzene to dissolve the sections completely
Implementation Method 3
mixing and centrifuging, discarding dimethylbenzene
Data Source
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AI summary
A method for preparing a liquid-state dropping or coating pathological quality control product, and uses thereof. In one aspect, the provided preparation method comprises the following steps: selecting and determining a reference material having a reference value, and processing the reference material; adding the processed reference material into an ethanol solution and storing the ethanol solution for standby use, the amount of the added ethanol solution being determined by the amount of a precipitate; and performing positive or negative reference setting by means of a dropping or coating manner. The pathological quality control product is a small-tissue slice suspension solution or homogenate, a cell or culture cell slice suspension solution or homogenate, or culture cell suspension solution or homogenate. In another aspect, also provided are uses of the liquid-state dropping or coating pathological quality control product as a positive or negative reference material in immunohistochemistry, in-situ hybridization, special stain and stain inspections of other tissues, and uses of the liquid-state dropping or coating pathological quality control product as a standard quality control product in pathological intra-room quality control and inter-room quality control.