Decellularized Liver ECM for Tissue Repair
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Solution Overview
Problem
Existing decellularization processes for deriving regenerative tissue materials from animal liver ECM struggle to maintain the natural collagen structure and bioactivity due to the difficulty in removing cells without damaging the ECM, often resulting in reduced protein content and effectiveness for tissue repair.
Innovation Solution
A method involving mechanical pressing and controlled chemical processing is employed to decellularize porcine liver ECM, retaining the collagen morphology and maintaining high levels of proteins and bioactive components, thereby forming a regenerative tissue repair product with enhanced structural integrity and bioactivity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If harsh chemical processes are used to remove cells from liver ECM, then cell removal efficiency is improved, but protein content and ECM effectiveness are reduced
Solution Approach 1:
The patent modifies the chemical parameters by using milder detergents (Triton X-100, SDS) at controlled concentrations and temperatures, combined with mechanical pressing forces, to achieve effective decellularization while preserving protein content. This resolves the contradiction by changing the chemical action parameters from harsh to controlled conditions.
Solution Approach 2:
The patent applies mechanical pressing before and during chemical treatment to facilitate cell removal. This preliminary and concurrent mechanical action enhances the effectiveness of milder chemical processes, enabling complete decellularization without requiring harsh chemicals that would damage proteins.
2Productivity
If mechanical pressing is applied during decellularization, then cell removal is improved, but collagen structure may be damaged
Solution Approach 1:
The patent applies mechanical pressing at multiple stages (before, during, and after chemical treatment) with varying forces. This partial application of mechanical force at different times achieves complete cell removal while allowing the collagen structure to recover and maintain its integrity between pressing cycles.
Solution Approach 2:
The patent uses chemical treatments (detergents) as a cushioning agent that weakens cell adhesion before mechanical pressing, reducing the force needed during pressing and thereby protecting the collagen structure from damage while still achieving effective cell removal.
3Productivity
If extended chemical treatment time is used, then cell removal is improved, but bioactivity of ECM is reduced
Solution Approach 1:
The patent applies mechanical pressing before chemical treatment to remove loosely attached cells, reducing the required chemical treatment time. This preliminary mechanical action prevents prolonged chemical exposure that would otherwise be needed to achieve complete decellularization, thereby preserving ECM bioactivity.
Solution Approach 2:
The patent uses periodic cycles of chemical treatment followed by mechanical pressing and rinsing. This periodic action achieves complete cell removal through repeated mild treatments rather than one extended harsh treatment, maintaining ECM bioactivity by limiting cumulative chemical exposure time.
Data Source
AI summary
A regenerative tissue repair product and method of making includes a decellularized extracellular matrix derived from animal liver. The decellularized extracellular matrix retains the collagen morphology of the animal liver prior to decellularization and having less than 500 ng DNA/mg dry extracellular matrix, greater than 760 microgram total collagen per milligram, the collagen comprising soluble and insoluble collagen, and greater than 1.6 μg glycosaminoglycans per milligram of dry extracellular matrix.