Liver Progenitor Cell Conditioned Medium for Regenerative Therapy

Resolve Bottlenecks,
Find Innovative SolutionsGenerate Solutions

Solution Overview

Problem

Current stem cell preparations for regenerative medicine, particularly those used in treating fulminant hepatic failure, face challenges due to immune reactions and potential cancer risks, and existing devices for bioartificial liver support lack sufficient efficacy and reliability.

Innovation Solution

A cell-free conditioned medium derived from a liver progenitor cell line, specifically a non-oval human liver pluripotent progenitor cell line, is used, which is shown to exert regenerative effects on the liver and kidney, avoiding the drawbacks of cell-based treatments by containing cytokines, chemokines, and growth factors.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If stem cell preparations are used for regenerative medicine, then regenerative effect on tissues is achieved, but immune reactions and potential cancer risks occur

Engineering Contradiction:

Improvesafety of treatmentVSAvoidimmune reactions and cancer risks

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent extracts the beneficial regenerative factors (cytokines, chemokines, growth factors) from the stem cell system and isolates them in conditioned medium, separating the therapeutic effect from the harmful cellular components that cause immune reactions and cancer risks

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The patent creates a cell-free copy of the stem cell therapeutic effect by collecting and analyzing the secreted molecules in conditioned medium, reproducing the regenerative benefits without using actual living stem cells that pose safety risks

Inventive Principle:

Principle #26Copying

2Reliability

If cell-based treatments are used, then regenerative effects are achieved, but immune reactions are caused

Engineering Contradiction:

Improveefficacy of treatmentVSAvoidimmune reactions

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent uses conditioned medium as an intermediary that carries the regenerative signal from stem cells to damaged tissues, mediating the therapeutic effect without requiring direct introduction of foreign cells that would trigger immune responses

Inventive Principle:

Principle #24Intermediary (Mediator)

3Reliability

If bioartificial liver support devices are used, then liver failure treatment is attempted, but sufficient efficacy and reliability are not achieved

Engineering Contradiction:

Improvetreatment reliabilityVSAvoidtreatment efficacy

Core Design Contradiction:

ReliabilityVSProductivity

Solution Approach 1:

The patent changes the fundamental parameter of the treatment from cell-based to cell-free, using concentrated conditioned medium with defined molecular compositions to achieve both high efficacy and reliability in treating liver failure without the limitations of previous devices

Inventive Principle:

Principle #35Parameter changes

Data Source

PatentUS10456425B2Conditioned medium of liver progenitor cells

Publication Date: 2019.10.29 FRESENIUS MEDICAL CARE DEUTSCHLAND GMBH

AI summary

The invention is in the field of regenerative medicine. It has been found that the conditioned medium of non-oval pluripotent liver progenitor cells exerts a tissue regenerating effect. A preparation of the cell free conditioned medium is therefore useful in the treatment of injury and organ failure, preferably liver and/or injury or failure.