Automated Living Cell Separation System with Impurity Ratio Analysis
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Solution Overview
Problem
Current methods for separating CD 56 positive cells, such as myoblast cells, from skeletal muscle tissue are labor-intensive, vary in efficiency among workers, and require manual mincing and enzymatic degradation, making it difficult to achieve stable and reliable separation.
Innovation Solution
A system that includes a mincing unit, a measurement unit to acquire tissue parameters, and an analysis unit to calculate the ratio of impurities to living cells, allowing for automated and precise separation by determining the optimal mincing parameters and removing impurities based on calculated ratios.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If manual mincing and enzymatic degradation are used to separate CD 56 positive cells from skeletal muscle tissue, then the separation process can be performed, but the operation is complicated, takes a long time, and shows high variability among workers
Solution Approach 1:
The patent replaces manual mechanical mincing with an automated mincing machine that uses controlled mechanical forces to disrupt muscle fibers. This substitution eliminates the variability and complexity associated with manual operations while maintaining effective tissue disruption for cell separation.
Solution Approach 2:
The patent implements automated control of mincing parameters such as cutting speed, blade configuration, and processing time. By precisely controlling these parameters, the system achieves consistent separation results across different operators while reducing overall process complexity through standardization.
2Productivity
If manual mincing is performed to separate living cells, then the process can be completed, but the determination depends on worker intuition causing variation in recovered cell numbers
Solution Approach 1:
The patent incorporates measurement units that monitor tissue parameters during mincing and provide feedback to the control system. This feedback mechanism enables real-time adjustment of mincing parameters to optimize cell recovery consistency while maintaining simple automated operation without requiring worker intuition.
Solution Approach 2:
The system performs self-optimization by automatically adjusting mincing parameters based on measured tissue characteristics. The automated system serves itself by making real-time decisions about processing conditions, eliminating the need for human judgment while ensuring consistent productivity.
3Reliability
If skeletal muscle tissue is minced to disrupt muscle fibers and loosen basement membrane, then CD 56 positive cells can be separated, but the process requires precise control to avoid destroying cells
Solution Approach 1:
The patent uses dynamically adjustable mincing parameters that can be modified in real-time based on tissue characteristics and separation progress. The system transitions between different mincing intensities and modes to achieve adequate tissue disruption while preserving cell integrity, reducing control complexity through automated adaptation.
Solution Approach 2:
The system performs preliminary assessment of tissue characteristics before initiating mincing and pre-configures optimal parameters. By preparing the mincing conditions in advance based on measured tissue properties, the system ensures cell integrity while simplifying control requirements through pre-programmed optimization.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This system enables simple, reliable, and automated separation of living cells, reducing labor and variability among workers, while ensuring efficient recovery of CD 56 positive cells.
Implementation Method 1
a measurement unit that measures a force applied to the living body-derived tissue
Data Source
AI summary
A system that easily and stably separates various living cells from a living body-derived tissue, the system including: a mincing unit that minces the living body-derived tissue based on a parameter; a measurement unit that acquires information regarding the living body-derived tissue being minced; and an analysis unit that calculates a ratio of impurities to the living body-derived tissue being minced from the information acquired by the measurement unit. Methods for separating various living cells from a living body-derived tissue are also disclosed.