LNTII Crystalline Form B for Stable Large-Scale Production
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Solution Overview
Problem
Existing methods for synthesizing lacto-N-triose II (LNTII) result in amorphous powders, which are unstable, energy-intensive, and costly, posing challenges for large-scale industrial production and long-term storage.
Innovation Solution
A new crystalline form of LNTII, referred to as crystalline form B, is developed with specific X-ray diffraction peaks, prepared by controlled cooling and crystallization using ethanol as a solvent, achieving stable and efficient large-scale production.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If spray-drying is used to obtain solid HMOs, then production efficiency is improved, but energy consumption increases and material loss occurs
Solution Approach 1:
The patent changes the physical state parameter from amorphous solid (obtained by spray-drying) to crystalline solid (obtained by cooling crystallization). This parameter change allows the product to be obtained through a different mechanism that avoids the high energy consumption and material loss associated with spray-drying, while still maintaining production efficiency
Solution Approach 2:
The patent utilizes phase transition from solution to crystalline solid through controlled cooling. The solution is cooled to a temperature where crystallization occurs, forming stable crystalline HMOs. This phase transition approach replaces the spray-drying process, eliminating the associated energy waste and material loss while maintaining productivity
2Ease of manufacture
If amorphous powder is obtained through conventional methods, then production process is simple, but stability is poor and shelf life is limited
Solution Approach 1:
The patent changes the structural parameter from amorphous to crystalline form through controlled cooling crystallization. This parameter change significantly improves product stability and shelf life while keeping the production process relatively simple through straightforward cooling and filtration steps
Solution Approach 2:
The patent employs preliminary action by pre-cooling the solution to a specific temperature range before crystallization occurs. This preliminary cooling step prepares the solution for controlled crystal formation, ensuring stable crystalline structure development while maintaining process simplicity
3Reliability
If conventional synthesis methods are used, then production cost is high, but product stability is insufficient for long-term storage
Solution Approach 1:
The patent utilizes phase transition from solution to crystalline solid through controlled cooling. This phase transition approach produces stable crystalline HMOs that are suitable for long-term storage, while the process is more cost-effective than conventional methods due to reduced energy consumption and material loss
Solution Approach 2:
The patent converts the potentially harmful effect of simple cooling into a beneficial crystallization process. By controlling the cooling rate and temperature, the solution transforms from an unstable amorphous state to a stable crystalline state, improving both stability and cost-effectiveness simultaneously
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The crystalline form B of LNTII offers improved stability, reducing production costs and extending shelf life, making it suitable for industrial applications in dairy products, food, pharmaceuticals, and cosmetics.
Implementation Method 1
prepared by controlled cooling and crystallization using ethanol as a solvent
Implementation Method 2
with the characteristic diffraction peaks at positions of 2θ values (2θ±0.2°) of diffraction angles of 3.98, 6.98, 8.30, 9.60, 18.68, 19.25, 19.89 and 20.72 in the powder X-ray diffraction pattern
Data Source
AI summary
A crystal of lacto-N-triaose, and belongs to the technical field of separation and purification. The technical solution is a crystalline form B of LNTII, which crystalline form has characteristic diffraction peaks at positions of 2θ values (2θ±0.2°) of diffraction angles of 3.98, 6.98, 8.30, 9.60, 18.68, 19.25, 19.89 and 20.72 in the powder X-ray diffraction pattern. Provided is a new crystalline form of lacto-N-triaose, thereby achieving the iteration of a technique for preparing LNTII and providing a reliable technique for industrial large-scale production.