Low-Reversion Tobacco Breeding via DNA Marker Selection
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Solution Overview
Problem
Tobacco breeding methods struggle to consistently produce low reversion type low nornicotine content tobacco varieties without genetic modification techniques, as high nornicotine type converters can still appear over generations, and genetic modifications often introduce undesired background mutations.
Innovation Solution
Selecting tobacco plants based on specific genomic DNA sequences linked to the Matsukawa (Kanto) variety, which are less likely to produce high nornicotine type converters, using markers such as SNV and InDel markers to identify and breed plants with the desired low reversion type low nornicotine character.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If conventional tobacco breeding methods are used to produce low nornicotine content varieties, then low nornicotine content can be achieved initially, but high nornicotine type converters reappear over generations
Solution Approach 1:
The patent applies preliminary action by using DNA markers (SNV and InDel markers) to identify and select plants carrying the desired low nornicotine content genetic traits at an early stage. This marker-assisted selection enables breeders to pre-screen and select plants with the stable low nornicotine content genotype before actual breeding, preventing the reappearance of high nornicotine converters in subsequent generations.
2Stability of the object's composition
If genetic modification techniques are used to produce low nornicotine content tobacco plants, then stable low nornicotine content can be achieved, but undesired background mutations are introduced
Solution Approach 1:
The patent extracts and utilizes specific DNA markers (SNV and InDel markers) that are naturally associated with low nornicotine content in the tobacco genome. By selecting plants based on these naturally occurring markers rather than introducing foreign genes, the method achieves stable low nornicotine content without the harmful background mutations that come with genetic modification techniques.
3Measurement precision
If marker-assisted selection is used to identify low reversion type plants, then selection precision is improved, but detection complexity increases
Solution Approach 1:
The patent employs simple and cost-effective marker detection methods for SNV and InDel markers that can be performed using basic molecular biology techniques. These markers serve as disposable, easy-to-detect genetic indicators that provide high selection precision without requiring complex or expensive detection equipment, making the process accessible for routine breeding programs.
Data Source
AI summary
Provided is a method for producing a low reversion type low nornicotine content tobacco plant line without use of a genetic modification technique or a factitious mutant preparation technique. Also provided is a tobacco plant. In the method in accordance with this disclosure, a variety-specific DNA sequence that links with a low reversion type low nornicotine character is determined, and selection is carried out with use of the sequence as an index.