Carotenoid Agent Preparation via Low-Temperature Solvent Dissolution

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Solution Overview

Problem

Existing preparation methods for carotenoids face challenges in preventing isomerization reactions during dissolution in organic solvents, leading to low content of all-trans isomers and reduced biological activity in feed additive products.

Innovation Solution

A method involving mixing a suspended organic dispersion phase of carotenoid crystals with a first organic solvent in a spiral coil heat exchanger, followed by emulsification, homogenization, and spray granulation to form a stable carotenoid agent with a high content of all-trans forms, using specific temperature and solvent ratios, and antioxidants to minimize isomerization.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Ease of manufacture

If traditional high-temperature melting method is used to prepare carotenoid emulsion, then the carotenoid can be dissolved and emulsified, but the oxidation and deterioration process of carotenoids is accelerated and isomer content changes greatly, resulting in poor long-term preservation

Engineering Contradiction:
Improveemulsion preparationVSAvoidlong-term preservation
Core Design Contradiction:
Ease of manufactureVSReliability

Solution Approach 1:

The patent changes the temperature parameter from traditional high-temperature melting to low-temperature processing (below the melting point of carotenoid crystals), and changes the dissolution method from direct heating to solvent dissolution at low temperature, thereby avoiding thermal oxidation and isomerization while maintaining emulsification capability

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent introduces organic solvents (such as ethanol, isopropanol, acetone) as intermediaries to dissolve carotenoid crystals at low temperatures, replacing the traditional direct high-temperature melting method. The solvent acts as a mediator that enables dissolution without requiring high temperature, thus protecting carotenoid stability

Inventive Principle:
Principle #24Intermediary (Mediator)

2Productivity

If high-temperature and high-pressure superheated steam is used for dissolution, then carotenoid can be processed, but significant loss in carotenoid content occurs and all-trans form content drops to only 70%, with expensive equipment and complicated process requirements

Engineering Contradiction:
Improvedissolution efficiencyVSAvoidcarotenoid content
Core Design Contradiction:
ProductivityVSLoss of substance

Solution Approach 1:

The patent fundamentally changes the dissolution parameters from high-temperature steam (200°C) to low-temperature solvent dissolution (below carotenoid melting point), and changes the pressure condition from high-pressure steam to atmospheric or mild pressure solvent system, thereby eliminating thermal degradation and isomerization while maintaining dissolution efficiency

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent replaces the mechanical/thermal system of high-temperature steam dissolution with a chemical dissolution system using organic solvents at low temperature, substituting thermal energy with chemical solvation to achieve dissolution without thermal damage

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

3Quantity of substance

If carotenoid is dissolved in organic solvent traditionally, then dissolution occurs, but isomerization reactions happen easily leading to low content of all-trans isomers and reduced biological activity

Engineering Contradiction:
ImprovedissolutionVSAvoidisomer composition
Core Design Contradiction:
Quantity of substanceVSStability of the object's composition

Solution Approach 1:

The patent changes the temperature parameter to low temperature (below carotenoid melting point) during solvent dissolution, and controls the residence time in the dissolution system, thereby preventing thermal and temporal conditions that promote isomerization while maintaining effective dissolution

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent takes preliminary protective actions by using low-temperature solvent dissolution to prevent isomerization before it can occur, and by quickly proceeding to emulsification and drying steps to remove solvents, thereby preventing subsequent isomerization reactions that would reduce all-trans content

Inventive Principle:
Principle #9Preliminary anti-action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The method achieves a carotenoid agent with a content of all-trans forms greater than or equal to 90% and enhanced stability, maintaining high biological activity and reducing isomerization.

Implementation Method 1

mixing a suspended organic dispersion phase of carotenoid crystal with a first organic solvent in a spiral coil of a coil heat exchanger to form a mixed solution, with the carotenoid crystal in the suspended organic phase of the carotenoid crystal being dissolved in the first organic solvent to obtain an oil phase matrix of the carotenoid dissolution solution, wherein the temperature inside the spiral coil is from 50°C to 70°C

Methodology Applied
Scientific EffectHeating: Heating

Implementation Method 2

the carotenoid crystal in the suspended organic phase of the carotenoid crystal being dissolved in the first organic solvent

Methodology Applied
Scientific EffectDissolution: Solvation

Implementation Method 3

mixing the aqueous phase matrix of the colloidal solution with the oil phase matrix of the carotenoid dissolution solution, and performing emulsification and dispersion as well as homogenization treatment successively to obtain a carotenoid nanodispersion

Methodology Applied
Scientific EffectEmulsification: Emulsion

Implementation Method 4

performing emulsification and dispersion as well as homogenization treatment successively to obtain a carotenoid nanodispersion

Methodology Applied
Scientific EffectHomogenization:

Implementation Method 5

removing the organic solvent from the carotenoid nanodispersion and concentrating the moisture to obtain a carotenoid emulsion; subjecting the carotenoid emulsion to spray granulation and drying successively to obtain the carotenoid agent

Methodology Applied
Scientific EffectEvaporation: Evaporation

Data Source

PatentEP4421064B1A preparation method of carotenoid agent
Publication Date: 2025.10.01 ZHEJIANG KEMING BIOPHARMACEUTICAL CO LTD

AI summary

The present disclosure provides a preparation method of carotenoid agent. The preparation method provided in present disclosure includes: mixing a suspended organic dispersion phase of a carotenoid crystal with a first organic solvent in a spiral coil of a coil heat exchanger to form a mixed solution, with the carotenoid crystal in the suspended organic phase of the carotenoid crystal being dissolved in the first organic solvent to obtain an oil phase matrix of the carotenoid dissolution solution, wherein the temperature inside the spiral coil is from 50°C to 70°C. In the present disclosure, the carotenoid crystal is dissolved in the first organic solvent at 50°C to 70°C, effectively avoiding the drawbacks of isomerization reaction of the carotenoid agent when it is dissolved in traditional preparation methods.