Soluble LR11 Immunoassay Using Sulfobetaine Surfactants
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Solution Overview
Problem
Conventional methods for immunologically assaying soluble LR11 in biological samples are cumbersome and impractical for clinical applications due to interference from unidentified substances, requiring extended reaction times and affecting measurement correlation.
Innovation Solution
Treatment of biological samples with sulfobetaine amphoteric or amidosulfobetaine amphoteric surfactants allows for shorter primary reaction times without compromising measurement accuracy, enabling efficient and sensitive quantitation of soluble LR11 levels.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional immunological assay methods are used to determine soluble LR11, then measurement accuracy is maintained, but assay time is excessively long and interfering substances affect results
Solution Approach 1:
The patent changes the chemical parameters of the assay system by introducing specific surfactants (Tween 20, Triton X-100, CHAPS, or N-acyl-N-methylglucamine) at optimized concentrations. These parameter changes modify the interaction between interfering substances and the antibody-antigen complex, allowing the assay to proceed rapidly (1 hour or less) while maintaining accuracy by eliminating interference effects.
2Productivity
If primary reaction time is shortened to increase productivity, then assay speed improves, but measurement correlation deteriorates
Solution Approach 1:
The patent introduces surfactants as intermediary substances that mediate the interaction between soluble LR11 and anti-LR11 antibodies. These intermediaries create a favorable microenvironment that accelerates the primary reaction kinetics while maintaining the specificity and correlation of measurements, enabling short reaction times without sacrificing measurement quality.
3Ease of operation
If simple immunological assay methods are used, then ease of operation improves, but interfering substances in biological samples cause incorrect determination
Solution Approach 1:
The patent modifies the assay parameters by incorporating surfactants into the assay buffer system. This parameter change maintains the simplicity of the immunological assay format while fundamentally improving accuracy by eliminating interference from biological sample components through the surfactant's ability to solubilize and neutralize interfering substances.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method significantly reduces assay time to one hour while maintaining the correlation and sensitivity of measurements, effectively eliminating the interference from substances present in the samples.
Implementation Method 1
the method comprises treating the sample with one or more surfactants selected from one or more sulfobetaine amphoteric surfactants and one or more amidosulfobetaine amphoteric surfactants
Implementation Method 2
employing an anti-soluble LR11 antibody... reaction between immobilized anti-soluble LR11 antibodies and soluble LR11
Data Source
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AI summary
To provide a method for assaying soluble LR11 in a biological sample, which method realizes a simple and accurate assay of soluble LR11 present in the sample by immunological means without requiring isolation of soluble LR11 from the biological sample (e.g., a serum sample). The method of the invention for immunologically assaying soluble LR11 present in a biological sample, characterized in that the method includes treating the sample with at least one surfactant selected from among one or more sulfobetaine amphoteric surfactants and one or more amidosulfobetaine amphoteric surfactants.