Soluble LR11 Quantification Using Surfactant Treatment
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Solution Overview
Problem
Current methods for quantifying soluble LR11 in biological samples, such as serum or plasma, face challenges due to interference from certain substances, making accurate measurement difficult and requiring complex separation processes.
Innovation Solution
The use of specific surfactants like polyoxyalkylene alkyl ether, polyoxyalkylene alkyl phenyl ether, alkyl glycoside, alkyl thioglycoside, acyl-N-methylglucamide, and cholic acid salts to treat samples before immunological measurement, allowing for direct and accurate quantification of soluble LR11 without complicated separation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If conventional immunological measurement methods are used to quantify soluble LR11 in serum or plasma, then measurement can be performed directly without separation, but accurate quantification cannot be achieved due to interference from certain substances in the sample
Solution Approach 1:
The patent introduces a specific antibody as an intermediary that selectively binds to soluble LR11 in the presence of interfering substances. This antibody acts as a mediator that distinguishes the target analyte from other components in the serum or plasma, enabling accurate measurement without complex separation steps. The antibody-LR11 complex can then be detected specifically, overcoming the interference problem while maintaining operational simplicity.
2Measurement precision
If separation processes are used to remove interfering substances before measurement, then measurement accuracy can be improved, but the operation becomes complicated and time-consuming
Solution Approach 1:
The patent extracts only the essential function of separation by using an antibody that specifically binds to soluble LR11, effectively 'taking out' the target analyte from the complex matrix of serum or plasma components. This selective extraction eliminates the need for complex physical separation processes while achieving the same goal of isolating LR11 from interfering substances, thereby maintaining accuracy without increasing process complexity.
3Measurement precision
If separation processes are used to remove interfering substances before measurement, then measurement accuracy can be improved, but measurement time and operational steps increase
Solution Approach 1:
The patent applies preliminary action by pre-coating the measurement plate or bead with a specific antibody against soluble LR11 before sample addition. This preliminary preparation ensures that when the sample is introduced, the antibody is already in position to immediately capture LR11, eliminating the need for subsequent separation steps. The interfering substances are effectively excluded from the outset, achieving accurate measurement rapidly without time-consuming separation procedures.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables convenient and sensitive measurement of soluble LR11 concentrations in body fluids, effectively overcoming interference from serum components and simplifying the quantification process.
Implementation Method 1
when a sample is treated with a certain kind of surfactant and then measurement is carried out based on an immunological measurement method, soluble LR11 in the sample can be quantified conveniently and accurately
Data Source
AI summary
Provided is a method for quantifying soluble LR11 in a biological sample such as serum by an immunological means conveniently and accurately without the need of carrying out any complicated separation manipulation. An immunological quantification method for soluble LR11 in a sample derived from a mammal, including a step of treating the sample with at least one surfactant selected from a group consisting of a polyoxyalkylene alkyl ether, a polyoxyalkylene alkyl phenyl ether, an alkyl glycoside, an alkylthio glycoside, an acyl-N-methylglucamide and a salt of cholic acid.