Leucine-Rich α2 Glycoprotein Composition for Alkaline pH Stability
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Solution Overview
Problem
Leucine-rich α2 glycoprotein (LRG) stability is compromised at pH levels below 7, leading to decomposition and loss over time, necessitating a solution for improved preservation.
Innovation Solution
A composition with a pH range of 7.8 to 9.0 and salt concentration of 300 mM to 600 mM, preferably using sodium salts, enhances LRG stability, allowing for accurate quantitative measurement.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If LRG is stored in a solution with pH less than 7, then the solution is stable and easy to prepare, but LRG is gradually decomposed and lost over time
Solution Approach 1:
The patent applies parameter changes by adjusting the pH of the storage solution from acidic (pH < 7) to alkaline (pH 7.8-9.0). This fundamental parameter change transforms the storage conditions to prevent LRG decomposition while maintaining solution stability. The specific pH range of 7.8-9.0 was determined to be optimal for preserving LRG integrity over extended storage periods.
2Stability of the object's composition
If the pH is adjusted to maintain LRG stability, then LRG decomposition is prevented, but the composition becomes more complex
Solution Approach 1:
Rather than adding complex stabilizing agents or multiple components, the invention simplifies the approach by changing the fundamental pH parameter to the alkaline range (7.8-9.0). This single parameter adjustment maintains LRG stability without requiring complex formulation strategies, buffer systems, or additional stabilizing compounds.
3Stability of the object's composition
If salt concentration is increased to 300-600 mM, then LRG stability is improved, but the solution becomes more concentrated and complex
Solution Approach 1:
The invention changes the salt concentration parameter to a specific high range (300-600 mM), which creates a physiological or near-physiological environment that stabilizes LRG. This parameter adjustment provides stability enhancement without requiring organic solvents, complex buffer systems, or multiple additives.
Data Source
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AI summary
An object is to provide a leucine-rich α2 glycoprotein composition comprising leucine-rich α2 glycoprotein and having excellent preservation stability. The object can be achieved by a leucine-rich α2 glycoprotein composition comprising leucine-rich α2 glycoprotein and having a pH of 7. 0 to 9.3.