LSPR Detection Chip Nano-Hole Metal Film for Extended Field Range
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Solution Overview
Problem
Conventional surface plasmon-field enhanced fluorescence spectroscopy (SPFS) is limited in detecting and measuring substances due to a narrow electric field enhancement region, restricting the types of fluorescent substances and antibodies that can be used, and is unable to detect proteins attached to cell surfaces.
Innovation Solution
A detection chip with a light-transmitting substrate and an LSPR structure that generates localized surface plasmon resonance, allowing for a wider electric field enhancement region, enabling detection of substances further from the substrate surface and on cell surfaces.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional SPR is used to generate electric field enhancement, then high sensitivity detection is achieved, but the electric field enhancement region is limited to about 200 nm from the substrate surface
Solution Approach 1:
The patent changes the physical parameters of the metal film by introducing nano-sized holes (10-100 nm diameter) into the metal film structure. This structural modification transforms the SPR mode, extending the electric field enhancement region from ~200 nm to several micrometers distance from the substrate surface, while maintaining high detection sensitivity
Solution Approach 2:
The patent creates a composite structure combining metal film with nano-sized holes, forming a novel sensor surface that integrates the plasmonic properties of metal with the field-enhancing effect of nanoscale cavities. This composite structure enables both high sensitivity and extended detection range
2Object-affected harmful factors
If the electric field enhancement region is narrowed to reduce noise, then floating substance noise is reduced, but the types of fluorescent substances and antibodies that can be used are limited
Solution Approach 1:
By modifying the metal film structure to include nano-sized holes, the patent extends the electric field enhancement region to several micrometers, allowing larger fluorescent substances and antibodies to be used while still maintaining noise rejection capabilities through the structured geometry
3Length of stationary object
If the electric field enhancement region is widened to detect substances further from the substrate, then more types of fluorescent substances and antibodies can be used, but noise from floating substances increases
Solution Approach 1:
The patent creates localized electric field enhancement at the nano-sized holes in the metal film, concentrating the enhanced field in specific regions while maintaining overall extended range. This localized enhancement strategy allows wide detection range without proportionally increasing noise from floating substances
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables the detection and measurement of substances with a wider range of fluorescent substances and antibodies, and the detection of proteins attached to cells or exosomes, improving the sensitivity and range of SPFS applications.
Implementation Method 1
an LSPR structure that generates localized surface plasmon resonance by light irradiation... the LSPR structure generates localized surface plasmon resonance, and an enhanced electric field region is formed on the liquid container side
Implementation Method 2
the fluorescent substance is excited by an electric field enhanced by SPR to emit fluorescence. By detecting this fluorescence, the detection target substance can be detected
Data Source
AI summary
A detection chip includes: a liquid container; and a light-transmitting substrate in which one of two surfaces facing each other faces an inside of the liquid container and an LSPR structure that generates localized surface plasmon resonance by light irradiation is disposed on the other surface of the two surfaces or in a region sandwiched between the one surface and the other surface.