Luminescent Particle Kit for Surface Plasmon Fluorescence Detection
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Solution Overview
Problem
Current fluorescence detection methods, particularly the SPF method, face challenges in measuring very small amounts of measurement target substances due to insufficient fluorescence intensity at high concentrations, leading to precision errors and issues with false positives from nonspecific adsorption, especially in biological samples.
Innovation Solution
A kit comprising a first particle labeled with a specific binding substance, a second particle without a label, and a flow channel, utilizing a luminescent labeled particle with an emission maximum wavelength of 680 nm or longer and high quantum yield, along with a substrate for surface plasmon fluorescence detection, to prevent false positives and enhance sensitivity across a wide concentration range.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If the SPF method is used to detect low concentrations of measurement target substance, then detection sensitivity is improved, but false positive occurs due to nonspecific adsorption
Solution Approach 1:
The patent introduces a blocking substance as an intermediary that specifically binds to the particle surface to prevent nonspecific adsorption of measurement target substances. This mediator blocks the harmful interaction between the particle surface and non-specifically binding substances, thereby reducing false positives while preserving the ability to detect low concentrations through specific binding events
Solution Approach 2:
The patent extracts and addresses the nonspecific adsorption problem by separately introducing a blocking substance that specifically targets and neutralizes the nonspecific binding sites on the particle surface. This allows the specific binding function to be preserved while the harmful nonspecific binding is removed or blocked
2Illumination intensity
If conventional fluorescent dyes are used, then fluorescence intensity is sufficient for detection, but measurement precision deteriorates at high concentrations due to insufficient fluorescence intensity
Solution Approach 1:
The patent changes the key parameter of fluorescence emission by selecting dyes with emission maximum wavelengths of 680 nm or longer. This parameter change in the emission wavelength range provides enhanced fluorescence intensity that maintains measurement precision across a wide concentration range from low to high concentrations
Solution Approach 2:
The patent applies local quality by selecting particles with specific fluorescent properties (emission wavelength ≥680 nm, high quantum yield) for use in specific detection scenarios. This localized optimization of particle properties ensures sufficient fluorescence intensity for high concentration measurements while maintaining sensitivity for low concentration detection
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution enables high-precision measurement of measurement target substances in biological samples by reducing nonspecific adsorption and noise, achieving accurate detection from low to high concentrations using surface plasmon fluorescence.
Implementation Method 1
a luminescent labeled particle having an emission maximum wavelength of 680 nm or longer and high quantum yield
Implementation Method 2
utilizing a luminescent labeled particle with an emission maximum wavelength of 680 nm or longer and high quantum yield, along with a substrate for surface plasmon fluorescence detection
Data Source
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AI summary
An object of the present invention is to provide a kit, a method, and a reagent which prevent the problem of false positive due to nonspecific adsorption, suppress the increase in noise to be generated, and are capable of achieving high-precision measurement of a measurement target substance in a wide concentration range from a low concentration to a high concentration. According to the present invention, there is provided a kit for measuring a measurement target substance, the kit including: a first particle having a label and modified with a first binding substance capable of specifically binding to a measurement target substance; a second particle having no label and modified with a second binding substance incapable of specifically binding to the measurement target substance; a flow channel for flowing the first particle and the second particle; and a substrate having a third binding substance capable of specifically binding to the measurement target substance or a substance capable of binding to the first binding substance, in which the first particle having a label is a luminescent labeled particle containing at least one kind of compound represented by Formula (1) and a particle. Each symbol in Formula (1) has the meaning described in the present specification.