Luminescent Sensor Pattern Correlation for Signal Noise Reduction
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Solution Overview
Problem
Affinity-based biosensors face challenges in achieving a high signal-to-noise ratio due to background signals interfering with the detection of luminescent targets, which hampers the accurate quantification of analytes.
Innovation Solution
A sensor device and method that utilize a processor to correlate measured luminescent patterns with reference patterns, employing techniques such as evanescent field generation, bleaching of background signals, and modulation of physical parameters to enhance the signal-to-noise ratio by distinguishing desired signals from background noise.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If affinity-based biosensors use fluorescent labels for signal transduction, then the detection capability is improved, but background signals interfere with the measurement precision
Solution Approach 1:
The patent segments the detection process into multiple temporal measurements and spatial pattern analyses. By dividing the measurement into reference pattern acquisition and sample pattern measurement, and analyzing spatial distributions of luminescent signals, the system separates specific binding signals from non-specific background signals, thereby improving measurement precision despite background interference
Solution Approach 2:
The patent converts background signals from harmful interference into useful information. By measuring background signals in reference regions and subtracting them from sample measurements, and by using the spatial distribution patterns of background signals to identify and eliminate false positives, the system transforms background noise into a means for improving measurement accuracy
2Measurement precision
If evanescent field excitation is used to excite fluorophores near the sensor surface, then the detection sensitivity is improved, but mobile background sources still generate interfering signals
Solution Approach 1:
The patent performs preliminary measurement of background signals using evanescent field excitation before introducing the sample. By acquiring reference patterns that capture mobile background sources under identical excitation conditions, the system establishes a baseline that can be subtracted from subsequent measurements, thereby maintaining detection sensitivity while eliminating mobile background interference
Solution Approach 2:
The patent implements feedback through continuous monitoring and comparison of measured patterns against reference patterns. By using the reference patterns obtained under evanescent field excitation as a feedback mechanism to identify and subtract mobile background sources from sample measurements, the system maintains high detection sensitivity while eliminating background interference
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The approach significantly improves the signal-to-noise ratio by effectively rejecting background signals and localizing mobile background sources, leading to more accurate quantification of luminescent targets and analytes.
Implementation Method 1
an evanescent field generating structure, wherein the light source is coupled to the evanescent field generating structure and wherein the evanescent field generating structure is adapted for generating an evanescent field at the surface
Implementation Method 2
a detector for obtaining an at least one-dimensional pattern of measured signals, wherein the detector is adapted for detecting the luminescence of the luminescent targets
Data Source
AI summary
A sensor device for quantifying luminescent targets configured in an at least one dimensional pattern. The sensor device comprises a detector for obtaining an at least one dimensional pattern of measured signals, wherein the detector is adapted for detecting the luminescence of the luminescent targets, resulting in a measured pattern. The sensor device moreover comprises a processor configured to correlate the measured pattern with at least one reference pattern, so as to generate a measurement signal representative for the quantification of luminescent targets. The at least one reference pattern is a recorded pattern or an expected pattern. A recorded pattern is a pattern which is obtained by the detector before the measured pattern is obtained.