Luminescent Substrate Micelle Formulation for Sustained Emission

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Solution Overview

Problem

Luminescence by luminescent enzymes exhibits a rapid decay in intensity immediately after the reaction, necessitating a method to improve luminescence sustainability.

Innovation Solution

A kit comprising a surfactant and an alcohol is used to react with a hydrophobic luminescent substrate of molecular weight 300 to 500, forming micelle particles that sustain luminescence.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Illumination intensity

If a luminescent enzyme and luminescent substrate are used for detection, then luminescence intensity is achieved, but luminescence sustainability deteriorates due to rapid decay after maximum intensity

Engineering Contradiction:

Improveluminescence intensityVSAvoidluminescence sustainability

Core Design Contradiction:

Illumination intensityVSDuration of action of stationary object

Solution Approach 1:

The invention changes the chemical parameters of the reaction system by introducing specific additives (surfactant and alcohol) to modify the luminescence reaction characteristics. This resolves the contradiction by altering reaction conditions to achieve both high intensity and sustained duration

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The invention introduces surfactant and alcohol as intermediary substances that mediate between the luminescent substrate and enzyme. These intermediaries form micelle structures that stabilize the reaction, allowing both high intensity and prolonged sustainability without direct modification of the enzyme or substrate

Inventive Principle:

Principle #24Intermediary (Mediator)

2Ease of manufacture

If conventional luminescence reaction conditions are used, then reaction simplicity is maintained, but luminescence half-life is short

Engineering Contradiction:

Improvereaction simplicityVSAvoidluminescence half-life

Core Design Contradiction:

Ease of manufactureVSDuration of action of moving object

Solution Approach 1:

The invention modifies reaction parameters by adding surfactant and alcohol to the conventional simple reaction system. This maintains ease of use while dramatically extending luminescence half-life from seconds to minutes

Inventive Principle:

Principle #35Parameter changes

3Illumination intensity

If hydrophobic luminescent substrates are used, then luminescence potential is improved, but solubility and reaction stability worsen

Engineering Contradiction:

Improveluminescence potentialVSAvoidreaction stability

Core Design Contradiction:

Illumination intensityVSStability of the object's composition

Solution Approach 1:

The invention uses surfactant as an intermediary that bridges the hydrophobic luminescent substrate and the aqueous reaction environment. The surfactant forms micelles that solubilize the hydrophobic substrate while maintaining reaction stability and enabling sustained luminescence

Inventive Principle:

Principle #24Intermediary (Mediator)

Data Source

PatentUS20250388810A1Kit for Sustaining Luminescence of Luminescent Substrate and Method for Sustaining Luminescence of Luminescent Substrate

Publication Date: 2025.12.25 SHIMADZU CORP

AI summary

A kit for sustaining luminescence of a luminescent substrate, the kit comprising a surfactant and an alcohol, wherein the luminescent substrate is a hydrophobic compound, and a molecular weight of the luminescent substrate is 300 or more and 500 or less.