Tissue Preservation Evaluation Using Luminescent Gene Markers
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Solution Overview
Problem
Current methods for evaluating the preservative effect of tissue preservation solutions are time-consuming, hindering the rapid development of new solutions.
Innovation Solution
Evaluating the preservative effect by immersing mammalian tissues with luminescence or fluorescence labeling genes, such as luciferase, in the preservation solution and measuring the resulting luminescence or fluorescence levels to assess the solution's effectiveness.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional tissue preservation evaluation methods are used (immersing isolated tissue and transplanting to animals or biochemical evaluation), then accurate preservative effect assessment is achieved, but evaluation time becomes extremely long
Solution Approach 1:
The patent introduces luminescence or fluorescence labeling genes as an intermediary indicator system. Instead of directly measuring tissue viability through complex transplantation or biochemical assays, the patent uses luminescence/fluorescence signal intensity as a mediator that correlates with tissue preservation quality, enabling rapid non-invasive assessment while maintaining evaluation accuracy
Solution Approach 2:
The patent replaces mechanical/surgical evaluation methods (tissue transplantation to animals) with optical detection methods (luminescence/fluorescence imaging). This substitution eliminates the need for animal transplantation procedures while providing real-time, quantitative assessment of preservative effects through non-invasive optical measurements
2Reliability
If conventional tissue preservation evaluation methods are used, then reliable preservative effect data is obtained, but development speed of new preservation solutions becomes slow
Solution Approach 1:
The patent introduces luminescence or fluorescence labeling genes into tissues before preservation evaluation. This preliminary genetic modification enables real-time monitoring of tissue preservation status during the evaluation process, allowing researchers to quickly screen and compare multiple preservation solutions without waiting for long-term transplantation outcomes, thereby accelerating development while maintaining data reliability
3Loss of time
If luminescence or fluorescence labeling genes are introduced into tissue, then real-time preservative effect evaluation is enabled, but measurement complexity increases
Solution Approach 1:
The patent utilizes the tissue's own luminescence or fluorescence signal (generated by the introduced labeling genes) as the evaluation indicator. The tissue itself serves as the light source, eliminating the need for external labeling procedures or complex preparation steps. This self-service approach simplifies the measurement process while enabling real-time, non-invasive assessment of preservative effects
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method allows for rapid and convenient evaluation of preservative effects, enabling faster development of tissue preservation solutions and simultaneous assessment across various tissues.
Implementation Method 1
measuring a luminescence or fluorescence level by the labeling gene in the tissue after immersion
Implementation Method 2
measuring a luminescence or fluorescence level by the labeling gene in the tissue after immersion
Data Source
AI summary
The present invention provides a method for evaluating preservative effect of a tissue preservation solution, comprising immersing a mammalian tissue introduced with a luminescence or fluorescence labeling gene in the tissue preservation solution, measuring a luminescence or fluorescence level by the labeling gene in the tissue after immersion, and evaluating the preservative effect of the tissue preservation solution based on the luminescence or fluorescence level.