Single Coagulation Reaction Lupus Anticoagulant Detection
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Solution Overview
Problem
Current methods for diagnosing lupus anticoagulant in antiphospholipid syndrome require multiple coagulation tests, increasing material and time requirements.
Innovation Solution
A method involving a single coagulation reaction with a lupus anticoagulant-sensitive APTT reagent, where coagulation time and specific parameters of the reaction curve are measured to detect lupus anticoagulant, allowing for its detection without the need for additional tests.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If multiple coagulation tests are performed to detect lupus anticoagulant, then detection reliability is improved, but time consumption and material usage increase
Solution Approach 1:
The patent combines the functions of multiple coagulation tests (screening test with low phospholipid concentration and confirmation test with high phospholipid concentration) into a single coagulation reaction by performing sequential measurements at different phospholipid concentrations within the same reaction mixture, thereby reducing time consumption and material usage while maintaining detection reliability
Solution Approach 2:
The single coagulation reaction system is designed to perform multiple functions: it can conduct both screening and confirmation of lupus anticoagulant presence by varying phospholipid concentration during the same reaction, making the system universal and eliminating the need for separate test procedures
2Reliability
If multiple coagulation tests are performed to detect lupus anticoagulant, then detection reliability is improved, but material consumption increases
Solution Approach 1:
The patent merges multiple test procedures into a single coagulation reaction by adding phospholipids at different concentrations sequentially during the same reaction, thereby reducing the total quantity of reagents including plasma samples, coagulation activators, and phospholipids required while maintaining the ability to reliably detect lupus anticoagulant
3Measurement precision
If multiple coagulation tests are performed to detect lupus anticoagulant, then detection accuracy is improved, but procedural complexity increases
Solution Approach 1:
The patent combines multiple test procedures into a single integrated coagulation reaction with automated sequential measurements at different phospholipid concentrations, reducing procedural complexity by eliminating the need for separate test setups, multiple reagent additions, and separate analysis steps while maintaining detection accuracy through the same reliable measurement principles
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method reliably differentiates lupus anticoagulant-containing samples from normal samples and others that prolong coagulation time, eliminating the need for a second coagulation reaction, with a sensitivity and specificity of 100% as demonstrated in the example.
Implementation Method 1
starting the coagulation reaction, measuring a measurement variable S of the reaction mixture over time (t), resulting in a function S(t) of time-dependent measurement values, and determining the coagulation time
Data Source
AI summary
The invention is in the field of coagulation diagnostics and relates to a method for detecting lupus anticoagulant.