Lycopene Micro-Capsule Powder Using Enzymatic Gelatin Hydrolysis

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Solution Overview

Problem

Lycopene micro-capsule powders prepared by existing methods often exhibit poor color and stability due to oxidation, light sensitivity, and the use of toxic solvents, limiting their application in functional products.

Innovation Solution

A method involving enzymatic hydrolysis of gelatin to reduce molecular weight, combined with disaccharides and polysaccharides, and natural antioxidants in an aqueous phase, followed by spray drying to create lycopene micro-capsules with improved color and stability, using a nano-grinder to achieve optimal particle size and mixing.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Stability of the object's composition

If conventional gelatin encapsulation is used, then lycopene micro-capsules can be formed, but the product exhibits dark color tone and poor color quality

Engineering Contradiction:
Improveencapsulation stabilityVSAvoidcolor brightness
Core Design Contradiction:
Stability of the object's compositionVSIllumination intensity

Solution Approach 1:

The patent changes the molecular weight parameter of gelatin from conventional high molecular weight (15000-250000 Da) to low molecular weight (5000-10000 Da) through enzymatic hydrolysis. This parameter change transforms the dense spatial network structure into a looser structure that allows better light transmission, thereby improving color brightness while maintaining encapsulation stability.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent creates a composite wall material system combining low molecular weight gelatin with disaccharides (such as sucrose or maltose) and polysaccharides (such as pullulan or chitosan). This composite structure provides both encapsulation stability and improved optical properties, achieving good color quality while protecting lycopene.

Inventive Principle:
Principle #40Composite materials

2Adaptability or versatility

If lycopene is exposed to environmental factors, then it can be used in functional products, but it undergoes oxidation and degradation by light, heat and oxygen

Engineering Contradiction:
Improveapplication scopeVSAvoidstability
Core Design Contradiction:
Adaptability or versatilityVSReliability

Solution Approach 1:

The patent uses a flexible micro-capsule shell formed by the gelatin-based wall material that encloses lycopene particles. This thin film structure provides physical barrier protection against oxygen and light while maintaining the adaptability for various functional product applications.

Inventive Principle:
Principle #30Flexible shells and thin films

Solution Approach 2:

The patent introduces antioxidants as intermediary substances within the wall material composition. These antioxidants act as mediators that actively prevent oxidation of lycopene by scavenging free radicals, thereby enhancing stability without limiting application scope.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Manufacturing precision

If toxic solvents are used in preparation, then lycopene can be fully dissolved and homogenized, but the product may cause harm to the human body

Engineering Contradiction:
ImprovehomogeneityVSAvoidtoxicity
Core Design Contradiction:
Manufacturing precisionVSObject-affected harmful factors

Solution Approach 1:

The patent replaces persistent toxic solvents with edible, biodegradable wall materials composed of gelatin, disaccharides, and polysaccharides. These food-grade materials provide the necessary dissolution and homogenization functions without leaving harmful residues, making the product safe for human consumption.

Inventive Principle:
Principle #27Cheap short-living objects (Disposable)

Solution Approach 2:

The patent changes the physical state and composition parameters of the wall material from synthetic solvent systems to natural polymer-based systems. This parameter change enables homogenization through enzymatic hydrolysis and physical mixing rather than chemical dissolution in toxic solvents.

Inventive Principle:
Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The method results in lycopene micro-capsule powder with a brighter, more vivid cherry red color and high stability to light, heat, and free radicals, maintaining over 97% lycopene content and color after one year, while being environmentally friendly and cost-effective for large-scale production.

Implementation Method 1

enzymatic hydrolysis of gelatin could decrease the molecular weight of gelatin

Methodology Applied
Scientific EffectEnzymatic hydrolysis: Enzyme

Implementation Method 2

adding protease under conditions of 55 to 65° C. and pH value of 7.5 to 8.5 for enzymatic hydrolysis

Methodology Applied
Scientific EffectProtease digestion: Hydrolysis

Implementation Method 3

lycopene is encapsulated in the wall material composed of the components

Methodology Applied
Scientific EffectEncapsulation: Physical Containment

Implementation Method 4

Micro-capsule technology can improve the stability of lycopene by effectively reducing the reaction of active substances to external environmental factors

Methodology Applied
Scientific EffectMicro-capsule formation: Microsphere

Implementation Method 5

grinding the emulsion to a particle size of less than 1 μm

Methodology Applied
Scientific EffectMechanical grinding: Abrasion

Implementation Method 6

spray drying

Methodology Applied
Scientific EffectSpray drying: Evaporation

Implementation Method 7

lycopene shows more significant effects in treatment and prevention of a variety of chronic diseases... lycopene has strong resistance to oxidation

Methodology Applied
Scientific EffectAntioxidation: Oxidation

Data Source

PatentUS11723879B2Lycopene micro-capsule powder and preparation method thereof
Publication Date: 2023.08.15 CHENGUANG BIOTECH GRP CO LTD

AI summary

The present invention discloses a method for preparing a lycopene micro-capsule powder, wherein lycopene is fully mixed with an aqueous phase to obtain a lycopene emulsion, and the emulsion is then ground to a particle size less than 1 μm, and spray dried. The aqueous phase comprises: a gelatin enzymatic hydrolysate with a molecular weight of 5,000-10,000 Da, disaccharide, polysaccharide, and an antioxidant. The disaccharide is one or more of sucrose, maltose and lactose; and the polysaccharide is one or more of pullulan and chitosan.