Multi-Part Lyophilization Container With Breathable Membrane

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Solution Overview

Problem

Current lyophilization methods for blood and blood products face challenges such as the use of breakable glass containers, prolonged drying times due to restricted vapor transmission, contamination risks, and inadequate breathable surface area, which complicate storage, transportation, and reconstitution, especially in resource-limited settings.

Innovation Solution

A multi-part lyophilization container with a non-breathable section for fluid storage and a breathable section for vapor flow, featuring a temporary occlusion zone that separates the sections to prevent contamination and enhance vapor flow, allowing for in situ lyophilization and easy reconstitution, and a method involving a temporary seal, freezing, and heat application under vacuum to facilitate efficient drying.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If a breathable membrane is used in a flexible pouch for lyophilization, then vapor transmission is enabled, but the membrane becomes wetted and fouled during the process, blocking vapor flow and increasing drying time

Engineering Contradiction:
Improvelyophilization rateVSAvoiddrying time
Core Design Contradiction:
ProductivityVSLoss of time

Solution Approach 1:

The container is divided into a liquid containment section and a vapor transmission section, separated by a partition barrier. This segmentation prevents the breathable membrane from contacting liquid directly while still enabling vapor transmission through the membrane to the condenser, resolving the fouling issue that blocks vapor flow.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

A partition barrier acts as an intermediary element between the liquid and the breathable membrane. This barrier allows vapor to pass through to the membrane for transmission to the condenser while preventing liquid from wetting and fouling the membrane, thus maintaining consistent vapor flow throughout the lyophilization process.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Reliability

If glass containers are used for lyophilization, then product integrity is maintained, but the containers are large and breakable, complicating storage and transportation

Engineering Contradiction:
Improveproduct integrityVSAvoidcontainer durability
Core Design Contradiction:
ReliabilityVSStrength

Solution Approach 1:

The patent replaces traditional rigid glass containers with a flexible pouch construction made of layered materials including a flexible outer layer and an inner liner. This flexible structure eliminates breakability while maintaining product integrity through the sealed, multi-layer design that protects the lyophilized product during storage and transportation.

Inventive Principle:
Principle #30Flexible shells and thin films

3Productivity

If current lyophilization devices are used, then vapor transmission occurs, but the total breathable surface area is inadequate, resulting in underperformance

Engineering Contradiction:
Improvelyophilization performanceVSAvoidbreathable surface area
Core Design Contradiction:
ProductivityVSArea of stationary object

Solution Approach 1:

The breathable membrane is configured to wrap around and contact multiple surfaces including the condenser and container walls in three-dimensional space. This spatial arrangement maximizes the effective breathable surface area beyond what a simple flat membrane would provide, significantly enhancing vapor transmission capacity and lyophilization performance.

Inventive Principle:
Principle #17Another dimension (Dimensionality change)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This solution reduces lyophilization time, minimizes contamination risks, and eliminates the need for cold chain management, making it suitable for resource-limited areas and providing a more stable and portable blood product.

Implementation Method 1

a breathable section (106) including a breathable membrane (108)... configured to accommodate only a gas

Methodology Applied
Scientific EffectVapor transmission: Permeation

Implementation Method 2

adding heat energy to the frozen liquid under vacuum

Methodology Applied
Scientific EffectSublimation: Sublimation

Implementation Method 3

adding heat energy to the frozen liquid under vacuum

Methodology Applied
Scientific EffectVacuum: Vacuum

Implementation Method 4

an occlusion zone (114)... encompassing a boundary bridging the non-breathable section and the breathable section

Methodology Applied
Scientific EffectOcclusion:

Data Source

PatentEP3694322B1Lyophilization container and method of using same
Publication Date: 2024.07.03 TERUMO BCT BIOTECHNOLOGIES LLC
  • EP3694322B1 patent drawingFigure 1A
  • EP3694322B1 patent drawingFigure 1B
  • EP3694322B1 patent drawingFigure 2A

AI summary

Provided is a multi-part lyophilization container (200) for lyophilizing a fluid, such as blood or blood components. The container includes a non-breathable section (202) including a port region (204), a breathable section (206) including a membrane (210), and an occlusion zone (216) encompassing a boundary between the two sections. The non-breathable section is configured to accommodate any of a liquid, a solid, a gas or combination thereof. The breathable section is configured to accommodate only a gas. A method of lyophilizing includes creating a temporary seal between the two sections, e.g. by manual clamping in the occlusion zone, inputting a liquid into the non-breathable section, freezing the liquid, removing the temporary seal to allow vapor transport between the two sections, and applying heat energy under vacuum. The container may then again be occluded in the occlusion zone, a permanent seam created, the container divided at the permanent seam and the breathable section discarded.