Lyophilized Mesenchymal Stem Cells Room Temperature Storage
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Solution Overview
Problem
Current methods for preserving mesenchymal stem cells, such as cryopreservation, face challenges in maintaining cell viability and functionality, especially during long-term storage and transportation, and lack standardization in isolation and culture techniques.
Innovation Solution
A lyophilized powder of mesenchymal stem cells is developed using a combination of lyoprotectants like trehalose, dextran, human serum albumin, and glycerol, which allows for storage at room temperature and maintains cell viability between 15% to 97%, facilitating easy transportation and distribution.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Duration of action of stationary object
If cryopreservation using liquid nitrogen is used to store MSCs, then long-term storage is achieved, but the method is complicated and expensive
Solution Approach 1:
The patent changes the storage parameter from ultralow temperature (liquid nitrogen, -196°C) to room temperature by using lyophilization. This parameter change simplifies the storage system while maintaining long-term preservation capability. The cells are freeze-dried to remove water, creating a stable dry state that can be stored at ambient conditions without specialized cryogenic infrastructure.
Solution Approach 2:
The patent replaces the mechanical cryopreservation system (requiring liquid nitrogen and complex cooling equipment) with a chemical lyophilization system. The cells are treated with cryoprotectants and freeze-dried, transforming them into a stable powdered form that can be stored简单地 at room temperature, eliminating the need for complex mechanical cooling systems.
2Duration of action of stationary object
If cryopreservation is used to preserve MSCs, then cell storage is achieved, but cell viability and functionality may be compromised during long-term storage and transportation
Solution Approach 1:
The patent applies preliminary action by treating cells with cryoprotectants (such as trehalose, dextran, glycerol, or polyethylene glycol) before lyophilization. This preliminary treatment protects the cell membranes and structures from damage during the freezing and drying process, ensuring high viability and functionality are maintained after storage and reconstitution.
Solution Approach 2:
The patent utilizes phase transitions in the lyophilization process: cells are first frozen (liquid to solid phase change), then water is removed through sublimation (solid ice to water vapor). This controlled phase transition process, combined with cryoprotectant treatment, preserves cell integrity and viability while enabling long-term storage at room temperature.
3Duration of action of stationary object
If conventional preservation methods are used for MSCs, then storage is achieved, but transportation and distribution become difficult and costly
Solution Approach 1:
The patent extracts water from the cell suspension through freeze-drying, removing the liquid phase that causes complexity in transportation. The resulting lyophilized powder form eliminates the need for specialized cryogenic shipping containers and cooling equipment, making transportation simple and cost-effective while maintaining cell viability.
4Manufacturing precision
If isolation and culture techniques for ASCs are standardized, then reproducibility is improved, but current techniques lack standardization
Solution Approach 1:
The patent establishes a universal lyophilization protocol that can be applied to mesenchymal stem cells from various sources (adipose tissue, bone marrow, umbilical cord, placenta). This standardized multi-functional approach enables consistent preservation and reproduction of cell properties across different cell types and laboratories, improving both standardization and reproducibility simultaneously.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The lyophilized mesenchymal stem cells demonstrate high cell viability and stability, enabling long-term preservation and cost-effective distribution, addressing the limitations of existing preservation methods.
Implementation Method 1
The present disclosure relates to a lyophilized powder of mesenchymal stem cells
Implementation Method 2
lyophilized powder of mesenchymal stem cells
Implementation Method 3
A lyophilized powder of mesenchymal stem cells is developed using a combination of lyoprotectants like trehalose, dextran, human serum albumin, and glycerol
Data Source
AI summary
The present disclosure belongs to the field of stem cell technology. Specifically, the present disclosure relates to a lyophilized powder of mesenchymal stem cells. More specifically, the present disclosure relates to a lyophilized adipose tissue derived mesenchymal stem cells. The present disclosure also relates to the advantageous use of lyophilized mesenchymal stem cells for long-term preservation, easy transportation and distribution of samples in a cost-effective way.