Lyophilized Stem Cell Exosomes Stabilization

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Solution Overview

Problem

Current anti-inflammatory treatments, such as immunosuppressive drugs and natural substance-based therapeutics, face challenges including adverse effects and limited efficacy, while stem cell-derived exosomes have stability issues when stored, affecting their anti-inflammatory potential.

Innovation Solution

A lyophilized formulation of stem cell-derived exosomes, stabilized with methionine, mannitol, trehalose, and optionally ascorbic acid and retinol, is developed for improved storage and anti-inflammatory efficacy, incorporated into an anti-inflammatory composition for pharmaceutical and cosmetic use.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If stem cell-derived exosomes are stored without lyophilization, then their biological activity is maintained, but their storage stability and shelf life deteriorate

Engineering Contradiction:

Improvestorage stabilityVSAvoidexosome structural integrity

Core Design Contradiction:

ReliabilityVSStability of the object's composition

Solution Approach 1:

The patent applies lyophilization (freeze-drying) to change the physical state of exosomes from liquid to solid form, removing water to prevent degradation while maintaining structural integrity through controlled parameter changes in temperature and pressure during the freezing and drying phases

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The patent uses protective agents (excipients) as intermediaries to prevent exosome damage during lyophilization. These agents form a protective matrix around exosomes during freezing and drying, preventing aggregation and structural collapse while maintaining biological activity

Inventive Principle:

Principle #24Intermediary (Mediator)

2Reliability

If conventional anti-inflammatory drugs are used, then inflammatory symptoms are controlled, but adverse effects such as skin atrophy and hypersensitivity reactions occur

Engineering Contradiction:

Improveanti-inflammatory efficacyVSAvoidadverse effects

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent extracts and utilizes only the beneficial anti-inflammatory components (exosomes containing anti-inflammatory cytokines and signaling molecules) while eliminating the harmful side effects associated with conventional drugs. The exosomes deliver therapeutic effects through natural biological mechanisms rather than pharmacological suppression

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The patent employs exosomes as temporary, biodegradable carriers that deliver anti-inflammatory signals and then naturally degrade, avoiding the cumulative toxicity and long-term adverse effects of persistent conventional medications

Inventive Principle:

Principle #27Cheap short-living objects (Disposable)

3Reliability

If natural substance-based therapeutics are used, then anti-inflammatory effects are achieved, but large amounts of the natural extract are needed due to low active ingredient content

Engineering Contradiction:

Improveanti-inflammatory effectVSAvoidamount of natural extract

Core Design Contradiction:

ReliabilityVSQuantity of substance

Solution Approach 1:

The patent extracts and isolates the specific anti-inflammatory active ingredients (exosomes) from stem cell secretomes, concentrating them to high purity. This eliminates the need to use large quantities of whole plant extracts while maintaining or enhancing therapeutic efficacy

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The patent creates a composite formulation combining exosomes with protective agents and buffering components, optimizing both stability and anti-inflammatory activity while using minimal amounts of the biologically active exosome material

Inventive Principle:

Principle #40Composite materials

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The lyophilized formulation effectively stabilizes stem cell-derived exosomes, exhibiting remarkable anti-inflammatory effects by significantly reducing inflammatory cytokine production, outperforming non-lyophilized exosomes in treating inflammatory diseases.

Implementation Method 1

a lyophilized formulation of stem cell-derived exosomes

Methodology Applied

Scientific EffectLyophilization: Freeze Drying

Data Source

PatentUS11529306B2Lyophilized formulation of stem cell-derived exosomes and anti-inflammatory composition including the same as active ingredient

Publication Date: 2022.12.20 EXOCOBIO INC

AI summary

The lyophilized formulation of stem cell-derived exosomes and the anti-inflammatory composition including the same as an active ingredient is able to stabilize stem cell-derived exosomes and exhibit excellent anti-inflammatory effects, and particularly, exhibit remarkable anti-inflammatory effects as compared with not-lyophilized stem cell-derived exosomes isolated and purified from conditioned media of stem cells. Therefore, the lyophilized formulation of stem cell-derived exosomes and the anti-inflammatory composition including the same as an active ingredient is able to effectively prevent, suppress, alleviate, ameliorate or treat inflammatory response or inflammatory diseases.