MADS-box polypeptides for maize grain yield enhancement
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Solution Overview
Problem
Current methods fail to effectively increase yield in plants, particularly in monocot crops like maize, where yield enhancement technologies are limited in efficacy and scope.
Innovation Solution
The use of polynucleotides encoding polypeptides with amino acid sequences at least 80% identical to specific MADS-box polypeptides (e.g., OsMADS18, SbMADS28) linked with heterologous promoters like GOS2, integrated into maize plants through genetic modification techniques such as CRISPR-Cas endonucleases, to enhance expression and activity, thereby increasing grain yield.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If current yield enhancement technologies are applied to monocot crops like maize, then some yield increase may be achieved, but the efficacy and scope are limited
Solution Approach 1:
The patent changes the molecular parameter by introducing heterologous MADS-box polypeptides with specific amino acid sequences (at least 80% identity to SEQ ID NOS: 1-10, 12-51) to alter the expression levels and activity of key regulatory proteins, thereby achieving reliable yield enhancement in maize
Solution Approach 2:
The patent uses heterologous promoters (such as GOS2 promoter) as intermediaries to drive the expression of MADS-box polypeptides, enabling controlled and effective gene expression that overcomes the limitations of current yield enhancement methods
2Productivity
If genetic modification techniques are used to increase polypeptide expression, then grain yield increases significantly, but the complexity of the genetic modification process increases
Solution Approach 1:
The patent extracts the key functional element (MADS-box polypeptide coding sequence) and places it under the control of a strong heterologous promoter, simplifying the genetic modification approach by focusing on the essential expression-driven yield enhancement mechanism
Solution Approach 2:
The patent modifies specific parameters of the genetic system by introducing polynucleotides encoding polypeptides with at least 80% amino acid identity to reference sequences, optimizing expression levels to achieve significant yield increases while maintaining manageable modification complexity
Data Source
AI summary
Provided are compositions comprising polynucleotides encoding polypeptides. Also provided are recombinant DNA constructs, plants, plant cells, seed, grain comprising the polynucleotides, and plants, plant cells, seed, grain comprising a genetic modification at a genomic locus encoding a polypeptide. Additionally, various methods of employing the polynucleotides and genetic modifications in plants, such as methods for modulating expression level in a plant and methods for increasing yield of a plant are also provided herein.