MADS-Box Polypeptides Enhance Maize Yield
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Solution Overview
Problem
Current methods fail to effectively increase yield in plants, particularly in monocot crops like maize, where yield enhancement technologies are limited and inefficient.
Innovation Solution
The use of polynucleotides encoding polypeptides with specific amino acid sequences, such as those found in SEQ ID NOS: 1-10 and 12-51, linked with regulatory elements like the GOS2 promoter, to enhance expression and activity of these polypeptides in plant cells, thereby increasing grain yield and photosynthetic activity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If current yield enhancement methods are used in monocot crops, then some yield increase may be achieved, but the methods are limited and inefficient in effectively increasing yield
Solution Approach 1:
The patent changes the molecular parameter of gene expression by introducing polynucleotides encoding specific MADS-box polypeptides with at least 80% amino acid sequence identity to SEQ ID NOS: 1-10 and 12-51. This molecular-level parameter change transforms the effectiveness of yield enhancement methods in monocot crops, achieving reliable grain yield increases where previous methods failed.
2Productivity
If polynucleotides encoding MADS-box polypeptides are introduced to increase grain yield, then productivity improves, but the complexity of the genetic modification process increases
Solution Approach 1:
The patent uses copying by introducing polynucleotides that encode polypeptides with at least 80% amino acid sequence identity to reference sequences (SEQ ID NOS: 1-10 and 12-51). Instead of creating entirely new complex genetic systems, the invention copies and adapts existing functional MADS-box gene sequences from related organisms, simplifying the genetic modification process while achieving reliable yield enhancement in monocot crops.
3Productivity
If targeted genetic modifications are made to increase polypeptide level and activity, then photosynthetic activity and yield increase, but the precision required for genetic modification increases
Solution Approach 1:
The patent applies parameter changes by specifying that encoded polypeptides must have at least 80% amino acid sequence identity to reference sequences, and by targeting specific expression levels and activities. This quantitative parameter approach enables precise control over photosynthetic activity enhancement while maintaining manageable genetic modification requirements, achieving both high productivity and acceptable precision in monocot crop improvement.
Data Source
AI summary
Provided are compositions comprising polynucleotides encoding polypeptides. Also provided are recombinant DNA constructs, plants, plant cells, seed, grain comprising the polynucleotides, and plants, plant cells, seed, grain comprising a genetic modification at a genomic locus encoding a polypeptide. Additionally, various methods of employing the polynucleotides and genetic modifications in plants, such as methods for modulating expression level in a plant and methods for increasing yield of a plant are also provided herein.