Water-Insoluble Magnesium Adsorbing Filter for Nucleic Acid Removal
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for purifying bio-pharmaceutical products, such as antibodies, are hindered by the presence of nucleic acids which increase viscosity, foul chromatography carriers, and decrease purification efficiency, and existing filters do not effectively remove these impurities without also adsorbing valuable antibodies.
Innovation Solution
A method involving the use of a water-insoluble magnesium compound, such as magnesium carbonate, magnesium hydroxide, or magnesium oxide, to adsorb nucleic acids while minimizing adsorption of antibodies, thereby facilitating the purification of bio-pharmaceutical products by reducing impurity levels through effective filtration.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional filtration and chromatography methods are used to purify antibodies, then purification can be achieved, but nucleic acids increase viscosity and foul chromatography carriers, decreasing purification efficiency
Solution Approach 1:
The patent applies preliminary action by introducing a nucleic acid removal step before the main purification process. The adsorbing filter removes nucleic acids from the liquid prior to chromatography, preventing viscosity increase and carrier fouling that would otherwise occur during subsequent purification steps.
Solution Approach 2:
The patent uses an adsorbing filter as an intermediary component between the crude liquid and the chromatography system. This filter selectively adsorbs nucleic acids while allowing antibodies to pass through, serving as a mediator that protects the downstream chromatography carriers from fouling.
2Quantity of substance
If existing filters are used to remove nucleic acids, then nucleic acid reduction may be achieved, but valuable antibodies are also adsorbed and lost
Solution Approach 1:
The adsorbing filter exhibits local quality through its selective adsorption properties. The filter material is designed or selected to have specific surface characteristics that create strong affinity for nucleic acids while maintaining low affinity for antibodies, enabling selective removal of impurities without losing the valuable product.
Solution Approach 2:
The patent applies parameter changes by optimizing the chemical and physical properties of the adsorbing filter material. By adjusting parameters such as surface charge, hydrophobicity, and pore size of the filter, the system achieves selective adsorption of nucleic acids over antibodies, controlling the interaction parameters to favor impurity removal while preserving product integrity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for the efficient removal of nucleic acids and other impurities, improving the purification process by maintaining the integrity of antibodies and reducing the load on subsequent chromatography steps, while being cost-effective and time-efficient.
Implementation Method 1
contacting the liquid with a water-insoluble magnesium compound to adsorb at least a part of the nucleic acid on the water-insoluble magnesium compound
Data Source
AI summary
The objective of the present invention is to provide a method for effectively and easily reducing an amount of a specific impurity in a liquid. The method for reducing an amount of a nucleic acid in a liquid according to the present invention is characterized in comprising the steps of contacting the liquid with a water-insoluble magnesium compound to adsorb at least a part of the nucleic acid on the water-insoluble magnesium compound. Also, the objective of the present invention is to provide an adsorbing filter useful for purifying a useful substance, such as an antibody and an antibody-like molecule, used as a purification material for effectively removing an impurity with easily maintaining the yield of the target substance due to excellent adsorption ability to a nucleic acid and low adsorption ability to an antibody, an antibody-like molecule or the like. The adsorbing filter is characterized in comprising the layer comprising a water-insoluble magnesium compound.