Magnetic Bead Nucleic Acid Extraction with Integrated Oscillation
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Solution Overview
Problem
Traditional nucleic acid extraction methods face challenges with poor time coordination and high costs due to row-by-row moving operations required for magnetic bead separation, leading to inefficient and uncontrollable magnetic adsorption processes.
Innovation Solution
A magnetic bead-based nucleic acid extraction apparatus incorporating a deep-well plate, a magnetic attraction mechanism, a position moving mechanism, and an oscillation mechanism, which enables precise up-and-down movement of magnetic bars for synchronized magnetic adsorption, oscillation, and solution transfer without the need for mechanical grippers, ensuring efficient and controlled nucleic acid extraction.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If row-by-row moving operation is used for magnetic adsorption, then magnetic bead separation can be completed, but time coordination is poor and cost increases
Solution Approach 1:
The patent combines the magnetic adsorption function and oscillation function into a single integrated device. The magnetic bar is positioned within the deep-well plate well, and the oscillation mechanism simultaneously performs both magnetic bead separation and solution mixing, eliminating the need for separate row-by-row operations and reducing overall processing time.
Solution Approach 2:
The magnetic bar serves multiple functions: it provides magnetic attraction for bead separation and simultaneously acts as an oscillation element for solution mixing. This multi-functional design allows simultaneous execution of separation and blending operations, improving time coordination and productivity.
2Reliability
If magnetic beads are adsorbed row by row, then separation can be achieved, but controllability of magnetic separation deteriorates
Solution Approach 1:
By integrating the magnetic bar directly into the well structure and combining it with the oscillation mechanism, the system achieves better control over magnetic separation. The magnetic bar remains stationary during oscillation, providing stable magnetic attraction while the oscillation enhances mixing, improving both reliability and ease of operation.
3Productivity
If mechanical gripper is used for transferring to oscillator, then row-by-row processing can be completed, but device complexity and cost increase
Solution Approach 1:
The patent eliminates the need for mechanical grippers and separate oscillators by integrating the oscillation mechanism directly into the magnetic separation device. The magnetic bar is positioned within the well and oscillates in place, removing the need for transferring samples between devices and simplifying the overall system architecture.
Solution Approach 2:
The integrated device performs multiple functions: magnetic bead separation, solution oscillation/mixing, and waste liquid removal, all within a single well. This eliminates the need for additional mechanical grippers and transfer mechanisms, reducing device complexity while maintaining productivity.
4Device complexity
If magnetic bar is fixed position, then结构简单 (structure is simple), but cannot achieve synchronized oscillation and magnetic adsorption
Solution Approach 1:
The magnetic bar is designed to be movable rather than fixed, allowing it to oscillate within the well. This dynamic positioning enables simultaneous magnetic adsorption and solution mixing, improving blending efficiency while maintaining relatively simple device structure through the integrated design.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The apparatus enhances blending efficiency and speed, reduces magnetic attraction time and cost, and maintains precise control over magnetic bead separation, allowing for simultaneous solution transfer and oscillation at the same position, thereby improving the overall nucleic acid extraction process.
Implementation Method 1
use the combination of a rod magnet and a disposable outer casing to complete the collection of the magnetic beads
Implementation Method 2
the apparatus for nucleic acid extraction needs to have an oscillating function
Data Source
AI summary
The present application provides a magnetic bead-based nucleic acid extraction apparatus including a well plate, a magnetic attraction mechanism, a position moving mechanism, and an oscillation mechanism, a supporting base, and a fixing base disposed on the supporting base. The magnetic attraction mechanism includes a magnetic bar fixing base and a magnetic bar movably disposed on the magnetic bar fixing base. The position moving mechanism includes a ball screw and a driving motor; two ends of the ball screw are respectively connected to the magnetic bar fixing base and the driving motor. The oscillation mechanism includes a carrier and a main oscillation unit connected to the carrier. The main oscillation unit includes a motor spindle, an eccentric column, and a connecting base.