Magnetic Cell Isolation Holder for Variable Particle Sizes

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Solution Overview

Problem

Magnetic particle-based cell isolation techniques face complexity due to varying magnetic properties of particles, requiring adjustments in magnetic field parameters and equipment settings for different particle sizes, which complicates the workflow and increases user interaction.

Innovation Solution

The use of specifically designed magnetic cell isolation holders that position particles within a magnetic field at locations corresponding to desired magnetic field characteristics, allowing for appropriate separation without altering magnetic field generator settings, and the provision of kits with multiple holders and particles optimized for different sizes and types.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If magnetic field parameters are adjusted for different particle sizes, then cell isolation effectiveness is improved, but device complexity and user interaction increase

Engineering Contradiction:

Improvecell isolation effectivenessVSAvoidworkflow complexity

Core Design Contradiction:

ReliabilityVSDevice complexity

Solution Approach 1:

The patent pre-configures multiple holders with passages positioned at specific locations within the magnetic field generator, where each position corresponds to optimized magnetic field parameters for different particle sizes. This preliminary arrangement eliminates the need for real-time parameter adjustments during cell isolation procedures.

Inventive Principle:

Principle #10Preliminary action

Solution Approach 2:

The invention changes the spatial position parameter of the holder passages within the magnetic field to accommodate different particle sizes. By positioning passages at different locations (e.g., closer to or farther from magnetic poles), the system optimizes magnetic field strength and gradient for various bead sizes without requiring active parameter adjustment.

Inventive Principle:

Principle #35Parameter changes

2Reliability

If magnetic field parameters are adjusted for different particle sizes, then cell isolation effectiveness is improved, but user interaction increases

Engineering Contradiction:

Improvecell isolation effectivenessVSAvoiduser interaction

Core Design Contradiction:

ReliabilityVSEase of operation

Solution Approach 1:

The system pre-configures multiple holders with passages positioned at specific locations within the magnetic field generator, where each position corresponds to optimized magnetic field parameters for different particle sizes. This preliminary arrangement eliminates the need for real-time parameter adjustments during cell isolation procedures.

Inventive Principle:

Principle #10Preliminary action

3Adaptability or versatility

If multiple holders with different passage positions are used, then adaptability to different particle sizes is improved, but device complexity increases

Engineering Contradiction:

Improveparticle size compatibilityVSAvoidholder configuration

Core Design Contradiction:

Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The magnetic field generator is designed to accommodate multiple holders with different passage positions, making it a universal system that can handle different particle sizes (e.g., Miltenyi nano-sized beads and Dynabeads) with a single device. The generator itself remains unchanged, while interchangeable holders provide the adaptability.

Inventive Principle:

Principle #6Universality (Multi-functionality)

Solution Approach 2:

The system segments the holder design into multiple versions, each with passages positioned at different locations within the magnetic field. This segmentation allows each holder to be optimized for specific particle sizes while maintaining compatibility with the same magnetic field generator through standardized coupling mechanisms.

Inventive Principle:

Principle #1Segmentation

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Enables efficient cell isolation with reduced user interaction and automated procedures, achieving high recovery and purity rates for different-sized particles, such as Miltenyi nano-sized and Dynabeads, without the need for frequent adjustments in magnetic field parameters.

Implementation Method 1

The target cells bound, via the antibodies or ligands, to the magnetic particles, are retained in the magnetic isolation device via magnetic force

Methodology Applied

Scientific EffectMagnetic force: Magnetism

Implementation Method 2

The passage of the first holder experiences a first magnetic field strength and a first magnetic field gradient within the magnetic field generated under the magnetic field parameters at the first location

Methodology Applied

Scientific EffectMagnetic field gradient: Magnetic Field

Data Source

PatentUS11332732B2Magnetic cell isolation techniques

Publication Date: 2022.05.17 GLOBAL LIFE SCIENCES SOLUTIONS USA LLC

AI summary

Provided are techniques that may include a disposable magnetic cell isolation holder having one or more passages that accommodate a magnetic retention material to facilitate magnetic cell isolation without adjustment of magnetic field parameters between isolation procedures using different magnetic particle sizes. When the magnetic cell isolation holder is coupled to a magnetic field generator, a first passage corresponding to a smaller particle size is positioned in a magnetic field where the magnetic field characteristics are different relative to a second passage, of a second holder or in the same holder, for use with another bead size.